Chronic Ethanol Administration Alters Hepatic Surface Membranes as Evidenced by Decreased Concanavalin A Binding

Jordan P. Metcalf, Carol A. Casey, Michael F. Sorrell, D. J. Tuma

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The effects of chronic ethanol administration on the hepatic surface membrane were examined. The binding of the lectin, concanavalin A (Con A), to isolated hepatocytes was used to ascertain changes in the hepatic plasma membrane, especially in regard to glycoprotein composition, due to chronic ethanol feeding. Hepatocytes, isolated from rats fed ethanol for 5 to 7 weeks, had a decreased ability to bind Con A when compared to hepatocytes from either the pair-fed controls or ad libitum chow-fed rats. Since decreased Con A binding was more apparent at high Con A concentrations, reduced lectin binding likely reflected changes in the composition of surface membrane glycoproteins in the livers of the ethanol-fed rats. When ethanol (50 mM) was added to the incubation medium containing hepatocytes from ethanol-fed rats, pair-fed controls, or chow-fed rats, no effects on Con A binding were observed. These results indicate that chronic ethanol administration induces changes in the oligosaccharide chains of plasma membrane glycoproteins in the liver. Such alterations may play a role in the pathogenesis of alcoholic liver disease.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume185
Issue number1
DOIs
StatePublished - May 1987

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Chronic Ethanol Administration Alters Hepatic Surface Membranes as Evidenced by Decreased Concanavalin A Binding'. Together they form a unique fingerprint.

  • Cite this