Chronic ethanol downregulates PKA activation and ciliary beating in bovine bronchial epithelial cells

T. A. Wyatt, J. H. Sisson

Research output: Contribution to journalArticlepeer-review

66 Scopus citations


Previously, we reported that ethanol (EtOH) stimulates a rapid increase in ciliary beat frequency (CBF) of bovine bronchial epithelial cells (BBEC). Agents activating cAMP-dependent protein kinase (PKA) also stimulate CBF. EtOH stimulates BBEC CBF through cyclic nucleotide kinase activation. However, EtOH-stimulated CBF is maximal by 1 h and subsides by 6 h, returning to baseline by 24 h. We hypothesized that the loss of EtOH-stimulated CBF was a result of downregulation of PKA activity. To determine the PKA activation state in response to EtOH, ciliated BBEC were stimulated for 0-72 h with various concentrations of EtOH and assayed for PKA. EtOH (100 mM) treatment of the cells for 1 h increased PKA activity threefold over unstimulated controls. PKA activity decreased with increasing time from 6 to 24 h. When BBEC were preincubated with 100 mM EtOH for 24 h, the stimulation of PKA by isoproterenol or 8-bromo-cAMP was abrogated. EtOH desensitizes BBEC to PKA-activating agents, suggesting that EtOH rapidly stimulates, whereas long-term EtOH downregulates, CBF via PKA in BBEC.

Original languageEnglish (US)
Pages (from-to)L575-L581
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Issue number3 25-3
StatePublished - 2001


  • Adenosine 3′,5′-cyclic monophosphate
  • Airway
  • Cyclic nucleotide
  • Down-regulation
  • Lung
  • cAMP-dependent protein kinase
  • β-agonist

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology


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