Chronic ethanol ingestion impairs TGF-α-stimulated receptor autophosphorylation

Dean J. Tuma, Sandra L. Todero, Mary Barak-Bernhagen, Carol A. Casey, Michael F. Sorrell

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The effects of chronic ethanol feeding on the binding of transforming growth factor-α (TGF-α) and TGF-α-stimulated receptor autophosphorylation were investigated in isolated rat hepatocytes. When hepatocytes were isolated from rats that were fed an ethanol liquid diet for 6-8 weeks, these cells exhibited a marked impairment of TGF-α-stimulated autophosphorylation of the receptor that binds this growth factor compared with hepatocytes from the pair-fed controls. This impaired autophosphorylation of receptor tyrosine residues was accompanied by significant decreases in the amount of surface- bound TGF-α. Immunoanalysis indicated no changes in receptor number, indicating that decreased receptor content was not responsible for decreased TGF-α binding in the hepatocytes from the ethanol-fed rats. In conclusion, chronic ethanol feeding reduced TGF-α binding to hepatocytes with a concomitant decrease in the ability of the receptor tyrosine kinase to autophosphorylate its tyrosine residues. These changes were not accompanied by decreased receptor protein content. These defects could lead to altered signal transduction and to impaired reparative and regenerative processes in the liver.

Original languageEnglish (US)
Pages (from-to)233-238
Number of pages6
JournalAlcohol
Volume15
Issue number3
DOIs
StatePublished - Apr 1 1998

Keywords

  • Autophosphorylation
  • EGF receptor
  • Ethanol
  • Liver
  • Receptors
  • Signal transduction
  • Transforming growth factor-α (TGF- α)

ASJC Scopus subject areas

  • Health(social science)
  • Biochemistry
  • Toxicology
  • Neurology
  • Behavioral Neuroscience

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