Clinical and pharmaceutical applications of affinity ligands in capillary electrophoresis: A review

Chenhua Zhang, Ashley G. Woolfork, Kyungah Suh, Susan Ovbude, Cong Bi, Marawan Elzoeiry, David S. Hage

Research output: Contribution to journalReview article

1 Scopus citations

Abstract

Affinity capillary electrophoresis (ACE) is a separation technique that combines a biologically-related binding agent with the separating power and efficiency of capillary electrophoresis. This review will examine several classes of binding agents that have been used in ACE and applications that have been described for the resulting methods in clinical or pharmaceutical analysis. Binding agents that will be considered are antibodies, aptamers, lectins, serum proteins, carbohydrates, and enzymes. This review will also describe the various formats in which each type of binding agent has been used in CE, including both homogeneous and heterogeneous methods. Specific areas of applications that will be considered are CE-based immunoassays, glycoprotein/glycan separations, chiral separations, and biointeraction studies. The general principles and formats of ACE for each of these applications will be examined, along with the potential advantages or limitations of these methods.

Original languageEnglish (US)
Article number112882
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume177
DOIs
StatePublished - Jan 5 2020

Keywords

  • Affinity capillary electrophoresis
  • Biointeraction studies
  • CE immunoassay
  • Chiral separations
  • Enzyme assays

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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