Clinical applications of capillary electrophoresis based immunoassays

Annette C. Moser, Corey W. Willicott, David S. Hage

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Immunoassays have long been an important set of tools in clinical laboratories for the detection, diagnosis, and treatment of disease. Over the last two decades, there has been growing interest in utilizing CE as a means for conducting immunoassays with clinical samples. The resulting method is known as a CE immunoassay. This approach makes use of the selective and strong binding of antibodies for their targets, as is employed in a traditional immunoassay, and combines this with the speed, efficiency, and small sample requirements of CE. This review discusses the variety of ways in which CE immunoassays have been employed with clinical samples. An overview of the formats and detection modes that have been employed in these applications is first presented. A more detailed discussion is then given on the type of clinical targets and samples that have been measured or studied by using CE immunoassays. Particular attention is given to the use of this method in the fields of endocrinology, pharmaceutical measurements, protein and peptide analysis, immunology, infectious disease detection, and oncology. Representative applications in each of these areas are described, with these examples involving work with both traditional and microanalytical CE systems.

Original languageEnglish (US)
Pages (from-to)937-955
Number of pages19
JournalELECTROPHORESIS
Volume35
Issue number7
DOIs
StatePublished - Apr 2014

Keywords

  • Affinity capillary electrophoresis
  • CE immunoassay
  • Capillary electrophoresis
  • Clinical chemistry
  • Immunoassay

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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