Cloning analysis of a disease resistance gene homolog from soybean

Bang jun Wang, Zhi Gang Zhang, Xue Gang Li, Yong Jun Wang, Chao Ying He, Jin Song Zhang, Shou Yi Chen

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Conserved domains e.g. nucleotide binding site (NBS) were found in several cloned plant disease resistance genes. Based on the NBS domain, resistance gene analogs (RGAs) have been isolated previously and were used as probes to screen a soybean (Glycine max L. Merr.) cDNA library. A full-length cDNA. KR3, was obtained by screening the library and rapid amplification of cDNA ends (RACE) method. Sequence analysis revealed that the cDNA is 2 353 bp in length and the open reading frame (ORF) codes for a polypeptide of 636 amino acids with a Toll-Interleukin-1 receptor (TIR) and a NBS domain. Sequence alignment showed that it was similar to N gene of tobacco. The phylogenetic tree analysis of R proteins with NBS from higher plants was performed. The KR3 gene has low copies in soybean genome and its expression was induced by exogenous salicylic acid (SA).

Original languageEnglish (US)
Pages (from-to)864-870
Number of pages7
JournalActa Botanica Sinica
Volume45
Issue number7
StatePublished - Jul 2003
Externally publishedYes

Keywords

  • Disease resistance gene homolog
  • Nucleotide binding site
  • Soybean
  • Toll-interleukin-1 receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

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  • Cite this

    Wang, B. J., Zhang, Z. G., Li, X. G., Wang, Y. J., He, C. Y., Zhang, J. S., & Chen, S. Y. (2003). Cloning analysis of a disease resistance gene homolog from soybean. Acta Botanica Sinica, 45(7), 864-870.