Cloning and analysis of the promoter activity of the human prostatic acid phosphatase gene

Stanislav Zelivianski, Dorothy Comeau, Ming Fong Lin

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Human prostatic acid phosphatase (PAP) has been proposed to be a prostate-epithelium differentiation antigen and its expression can be regulated by androgen. Nevertheless, the regulatory mechanism at the molecular level is not completely understood. In this communication, we demonstrated the tissue-specific expression of PAP in the normal prostate epithelium. Furthermore, results of nuclear run-on experiments indicated that androgen could regulate the transcriptional rate of the PAP gene. This mode of regulation was modulated by cell density. To investigate the transcriptional regulation, we cloned and characterized a 1.4-kilobase (kb) fragment of DNA that flanks the 5@? region of the PAP gene from LNCaP human prostate carcinoma cells. The results demonstrated that this 1.4-kb DNA fragment can drive a chloramphenicol acetyltransferase (CAT) gene expression in LNCaP cells. Also, the promoter activity was inversely correlated with the growth of those cells.

Original languageEnglish (US)
Pages (from-to)108-112
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume245
Issue number1
DOIs
StatePublished - Apr 7 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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