TY - JOUR
T1 - Cloning and analysis of the promoter activity of the human prostatic acid phosphatase gene
AU - Zelivianski, Stanislav
AU - Comeau, Dorothy
AU - Lin, Ming Fong
N1 - Funding Information:
We thank Fen-Fen Lin for preparing genomic DNA from LNCaP cells and critical comments from Dr. Prathibha Rao and Tzu-Ching Meng. This work was supported in part by grants from the National Cancer Institute (CA72274), Dean of the College of Medicine at UNMC for the Prostate Cancer Program, and the CaPCURE Foundation.
PY - 1998/4/7
Y1 - 1998/4/7
N2 - Human prostatic acid phosphatase (PAP) has been proposed to be a prostate-epithelium differentiation antigen and its expression can be regulated by androgen. Nevertheless, the regulatory mechanism at the molecular level is not completely understood. In this communication, we demonstrated the tissue-specific expression of PAP in the normal prostate epithelium. Furthermore, results of nuclear run-on experiments indicated that androgen could regulate the transcriptional rate of the PAP gene. This mode of regulation was modulated by cell density. To investigate the transcriptional regulation, we cloned and characterized a 1.4-kilobase (kb) fragment of DNA that flanks the 5@? region of the PAP gene from LNCaP human prostate carcinoma cells. The results demonstrated that this 1.4-kb DNA fragment can drive a chloramphenicol acetyltransferase (CAT) gene expression in LNCaP cells. Also, the promoter activity was inversely correlated with the growth of those cells.
AB - Human prostatic acid phosphatase (PAP) has been proposed to be a prostate-epithelium differentiation antigen and its expression can be regulated by androgen. Nevertheless, the regulatory mechanism at the molecular level is not completely understood. In this communication, we demonstrated the tissue-specific expression of PAP in the normal prostate epithelium. Furthermore, results of nuclear run-on experiments indicated that androgen could regulate the transcriptional rate of the PAP gene. This mode of regulation was modulated by cell density. To investigate the transcriptional regulation, we cloned and characterized a 1.4-kilobase (kb) fragment of DNA that flanks the 5@? region of the PAP gene from LNCaP human prostate carcinoma cells. The results demonstrated that this 1.4-kb DNA fragment can drive a chloramphenicol acetyltransferase (CAT) gene expression in LNCaP cells. Also, the promoter activity was inversely correlated with the growth of those cells.
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U2 - 10.1006/bbrc.1998.8386
DO - 10.1006/bbrc.1998.8386
M3 - Article
C2 - 9535792
AN - SCOPUS:0032492580
VL - 245
SP - 108
EP - 112
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -