Cloning and detection of DNA from a nonculturable plant pathogenic mycoplasma-like organism

Bruce C. Kirkpatrick, Drake C. Stenger, T. Jack Morris, Alexander H. Purcell

Research output: Contribution to journalArticle

117 Scopus citations

Abstract

The ability to detect, quantify, and differentiate nonculturable mycoplasma-like organisms (MLOs) would greatly facilitate epidemiological and taxonomical studies of this unique group of plant and insect pathogens. DNA isolated from extracts of insects infected with the Western X-disease MLO was cloned in Escherichia coli. X-disease-specific clones, when labeled and used as probes, readily detected X-disease MLOs in infected plants and insects but did not hybridize with DNA from healthy plants or insects, or from several other plant pathogenic MLOs or spiroplasmas. These methods provide both a sensitive diagnostic tool and a basis for genetically differentiating MLOs.

Original languageEnglish (US)
Pages (from-to)197-200
Number of pages4
JournalScience
Volume238
Issue number4824
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • General

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    Kirkpatrick, B. C., Stenger, D. C., Jack Morris, T., & Purcell, A. H. (1987). Cloning and detection of DNA from a nonculturable plant pathogenic mycoplasma-like organism. Science, 238(4824), 197-200. https://doi.org/10.1126/science.238.4824.197