Cloning and dosage compensation of the 6‐phosphogluconate dehydrogenase gene (Pgd+) of Drosophila melanogaster

Anthony G. Gutierrez, Alan C. Christensen, Jerry E. Manning, John C. Lucchesi

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Using a heterologous rat cDNA probe, we have identified a 14.7 kbp Drosophila melanogaster genomic clone containing the X‐linked gene Pgd+, which encodes the enzyme 6‐phosphogluconate dehydrogenase (6PGD). We used in situ hybridization to larval polytene chromosomes, a somatic transient expression assay for enzyme activity, and the rescue of the lethal Pgd phenotype by germline transformation to verify the identity of the gene. A 7.4 kbp fragment including the gene and approximately 1.2 kbp of upstream and 1.8 kbp of downstream sequences was relocated to autosomal ectopic sites by germline transformation; this transduced gene exhibits levels of enhanced activity in males comparable to those of the indigenous gene at its normal X chromosome locus. We conclude that the sequences responsible for dosage compensation of Pgd+ are included in this fragment.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalDevelopmental Genetics
Volume10
Issue number3
DOIs
StatePublished - 1989

Keywords

  • 6PGD
  • 6‐phosphogluconate dehydrogenase
  • X chromosome

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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