Abstract
Using a heterologous rat cDNA probe, we have identified a 14.7 kbp Drosophila melanogaster genomic clone containing the X‐linked gene Pgd+, which encodes the enzyme 6‐phosphogluconate dehydrogenase (6PGD). We used in situ hybridization to larval polytene chromosomes, a somatic transient expression assay for enzyme activity, and the rescue of the lethal Pgd− phenotype by germline transformation to verify the identity of the gene. A 7.4 kbp fragment including the gene and approximately 1.2 kbp of upstream and 1.8 kbp of downstream sequences was relocated to autosomal ectopic sites by germline transformation; this transduced gene exhibits levels of enhanced activity in males comparable to those of the indigenous gene at its normal X chromosome locus. We conclude that the sequences responsible for dosage compensation of Pgd+ are included in this fragment.
Original language | English (US) |
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Pages (from-to) | 155-161 |
Number of pages | 7 |
Journal | Developmental Genetics |
Volume | 10 |
Issue number | 3 |
DOIs | |
State | Published - 1989 |
Externally published | Yes |
Keywords
- 6PGD
- 6‐phosphogluconate dehydrogenase
- X chromosome
ASJC Scopus subject areas
- Genetics
- Developmental Biology
- Cell Biology