TY - JOUR
T1 - Cloning and expression of the Zymomonas mobilis 'production of ethanol' genes in Lactobacillus casei
AU - Gold, R. Shane
AU - Meagher, Michael M.
AU - Tong, Suxiang
AU - Hutkins, Robert W.
AU - Conway, Tyrrell
PY - 1996/10
Y1 - 1996/10
N2 - This study describes the expression of the Zymomonas mobilis genes coding for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) in Lactobacillus casei 686. To promote transcription, the promoter and ribosome binding site (RBS) from the Lactococcus lactus subsp. lactis-derived vector, pMGE36e, were inserted upstream of the pdc gene. The former sequences were positioned such that translation of pdc was coupled to translation of an 81- base pair open reading frame terminating within the pdc initiation site. The recombinant plasmid (pRSG02) was electroporated into L. casei, and transformants were obtained. Northern analysis confirmed the production of a 3.1-kb transcript corresponding to the predicted size of the PET operon. Western blot analyses revealed that the recombinant strain expressed both enzymes. The recombinant produced more than twice the ethanol produced by the parental L. casei strain.
AB - This study describes the expression of the Zymomonas mobilis genes coding for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) in Lactobacillus casei 686. To promote transcription, the promoter and ribosome binding site (RBS) from the Lactococcus lactus subsp. lactis-derived vector, pMGE36e, were inserted upstream of the pdc gene. The former sequences were positioned such that translation of pdc was coupled to translation of an 81- base pair open reading frame terminating within the pdc initiation site. The recombinant plasmid (pRSG02) was electroporated into L. casei, and transformants were obtained. Northern analysis confirmed the production of a 3.1-kb transcript corresponding to the predicted size of the PET operon. Western blot analyses revealed that the recombinant strain expressed both enzymes. The recombinant produced more than twice the ethanol produced by the parental L. casei strain.
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U2 - 10.1007/s002849900109
DO - 10.1007/s002849900109
M3 - Article
C2 - 8824172
AN - SCOPUS:0029797066
SN - 0343-8651
VL - 33
SP - 256
EP - 260
JO - Current Microbiology
JF - Current Microbiology
IS - 4
ER -