@article{45b497201e254a3e8657515718212fe4,
title = "Cloning and sequencing of the cDNA encoding human glutaredoxin",
abstract = "Glutaredoxin (thioltransferase) is a small, heat-stable protein, which is involved in thiol/disulfide exchange reactions. We have isolated a cDNA that encodes glutaredoxin from a human brain cDNA library. The encoded protein contains 106 amino acids with a calculated molecular mass of 11.76 kDa and an isoelectric point of 8.09. The amino acid sequence deduced from the cDNA is more than 80% identical to those of other mammalian glutaredoxins.",
keywords = "(Human), Glutaredoxin, Nucleotide sequence, Thioltransferase, cDNA cloning",
author = "Fernando, {M. Rohan} and Hideki Sumimoto and Hiroki Nanri and Kawabata, {Shun ichiro} and Sadaaki Iwanaga and Shigeki Minakami and Yasuyuki Fukumaki and Koichiro Takeshige",
note = "Funding Information: except that the former has no tryptophan residue but the latter contains two \[6\]T. he reason for this discrepancy is presently unknown. Although human glutaredoxin is purufied \[6-8\]a nd its cDNA is cloned as shown in this study, the precise role of the enzyme in human cells is not well understood, and little is known about regulation of the enzyme activity and the enzyme level. The cDNA for human glutaredoxin provides a powerful tool for further studies to address such questions. We thank Dr. Y. Misumi (Department of Biochemistry, Fukuoka University School of Medicine) and Dr. Y. Sakaki (Institute of Medical Science, University of Tokyo) for cDNA library, Dr. H. Sasaki (Institute of Genetic information, Kyushu university) and K. Nakashima (Department of Biology, Faculty of Science, Kyushu University) for technical advices and stimulating discussions and H. Furumi (Institute of Genetic information, Kyushu university) for oligonucleotide synthesis. This study was supported in part by grants from the Ministry of Education, Science and Culture of Japan.",
year = "1994",
month = jun,
day = "21",
doi = "10.1016/0167-4781(94)90019-1",
language = "English (US)",
volume = "1218",
pages = "229--231",
journal = "BBA - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier B.V.",
number = "2",
}