Cloning, characterization and expression of the gene coding for a cytosine-5-DNA methyltransferase recognizing GpC

Mai Xu, Michael P. Kladde, James L. Van Etten, Robert T. Simpson

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

A novel gene encoding a cytosine-5-DNA methyltransferase recognizing the dinucleotide GpC was cloned from Chlorella virus NYs-1 and expressed in both Escherichia coil and Saccharomyces cerevisiae. The gene was sequenced and a predicted polypeptide of 362 amino acids with a molecular weight of 41.903 kDa was identified. The protein contains several amino acid motifs with high similarity to those of other known 5-methylcytosine-forming methyltransferases. In addition, this enzyme, named M.CviPI, shares 66% identity and 76% similarity with M. CviJI, the only other cytosine-5-DNA methyltransferase cloned from a Chlorella virus. The short, frequently occurring recognition sequence of the new methyltransferase will be very useful for in vivo chromatin structure studies in both yeast and higher organisms.

Original languageEnglish (US)
Pages (from-to)3961-3966
Number of pages6
JournalNucleic acids research
Volume26
Issue number17
DOIs
StatePublished - Sep 1 1998

ASJC Scopus subject areas

  • Genetics

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