Cloning, characterization, and genetics of the juvinile hormone esterase gene from Heliothis virescens

L. G. Harshman, V. K. Ward, J. K. Beetham, D. F. Grant, L. J. Grahan, C. A. Zraket, D. G. Heckel, B. D. Hammock

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The gene for juvenile hormone esterase (JHE) was cloned from Heliothis virescens (Lepidoptera: Noctuidae). A genomic library was constructed from embryonic DNA and screened with a homologous N-terminal probe from the JHE cDNA. Five genomic clones were isolated and analyzed by dot blot hybridization using regions of the JHE cDNA as probes. Clone C hybridized to both 5′ and 3′ probes from the JHE cDNA, suggesting that clone C contains both ends of JHE gene. This was verified by sequencing the ends of the JHE gene from clone C using primers from both the 5′ and 3′ ends of the JHE cDNA. Additional sequencing and restriction mapping were used to characterized the gene. The gene is c. 8 kb long and contains four introns with consensus intron-exon junctions. One of the introns is relatively large (4 kb) and is situated near the extreme 5′ end of the gene. Genetic analysis of RFLP variation in interspecific and intraspecific crosses shows that the JHE locus is single-copy with no closely related paralogs and is autosomally encoded in Heliothis. Therefore the developmental pattern of expression of this gene and the previously documented sequence variation in cDNA clones is not explainable by reference to a JHE gene family with distinct structural loci for the different forms.

Original languageEnglish (US)
Pages (from-to)671-676
Number of pages6
JournalInsect Biochemistry and Molecular Biology
Volume24
Issue number7
DOIs
StatePublished - Jul 1994

Keywords

  • Heliothis virescens
  • Juvenile hormone esterase
  • Juvenile hormone esterase gene
  • Juvinile hormone

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

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