Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies

Medicine & Life Sciences

• Affinity Chromatography 10%
• antimicrobial peptide LL-37 100%
• Cyanogen Bromide 14%
• Databases 5%
• Dipeptides 12%
• Escherichia coli 41%
• formic acid 25%
• High Pressure Liquid Chromatography 8%
• Human Body 9%
• Infections 4%
• Isotope Labeling 86%
• Isotopes 10%
• Magnetic Resonance Spectroscopy 49%
• Mass Spectrometry 8%
• Membranes 5%
• Nitrogen-15 18%
• Organism Cloning 54%
• Peptides 37%
• Polyacrylamide Gel Electrophoresis 9%
• Proteins 10%
• Thioredoxins 12%