Cocrystallization studies of full-length recombinant butyrylcholinesterase (BChE) with cocaine

Oluwatoyin Ajibola Asojo; Oluyomi Adebola Asojo; Michelle N. Ngamelue; Kohei Homma; Oksana Lockridge

doi:10.1107/S1744309111004805

Cocrystallization studies of full-length recombinant butyrylcholinesterase (BChE) with cocaine

Oluwatoyin Ajibola Asojo, Oluyomi Adebola Asojo, Michelle N. Ngamelue, Kohei Homma, Oksana Lockridge

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Human butyrylcholinesterase (BChE; EC 3.1.1.8) is a 340 kDa tetrameric glycoprotein that is present in human serum at about 5 mg l^-1 and has well documented therapeutic effects on cocaine toxicity. BChE holds promise as a therapeutic that reduces and finally eliminates the rewarding effects of cocaine, thus weaning an addict from the drug. There have been extensive computational studies of cocaine hydrolysis by BChE. Since there are no reported structures of BChE with cocaine or any of the hydrolysis products, full-length monomeric recombinant wild-type BChE was cocrystallized with cocaine. The refined 3 Å resolution structure appears to retain the hydrolysis product benzoic acid in sufficient proximity to form a hydrogen bond to the active-site Ser198.

Original language	English (US)
Pages (from-to)	434-437
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	67
Issue number	4
DOIs	https://doi.org/10.1107/S1744309111004805
State	Published - Apr 2011

Keywords

BChE
cocaine hydrolysis
recombinant butyrylcholinesterase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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10.1107/S1744309111004805

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Cocrystallization studies of full-length recombinant butyrylcholinesterase (BChE) with cocaine. / Asojo, Oluwatoyin Ajibola; Asojo, Oluyomi Adebola; Ngamelue, Michelle N. et al.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 67, No. 4, 04.2011, p. 434-437.

Research output: Contribution to journal › Article › peer-review

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AU - Homma, Kohei

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Cocrystallization studies of full-length recombinant butyrylcholinesterase (BChE) with cocaine

Abstract

Keywords

ASJC Scopus subject areas

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