Codon Bias Can Determine Sorting of a Potassium Channel Protein

Anja J. Engel, Marina Kithil, Markus Langhans, Oliver Rauh, Matea Cartolano, James L. Van Etten, Anna Moroni, Gerhard Thiel

Research output: Contribution to journalArticlepeer-review

Abstract

Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K+ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins.

Original languageEnglish (US)
JournalCells
Volume10
Issue number5
DOIs
StatePublished - May 7 2021

Keywords

  • codon usage
  • dual sorting
  • effect of synonymous codon exchange
  • membrane protein sorting

ASJC Scopus subject areas

  • Medicine(all)

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