Collection of blood mononuclear cells by leukapheresis for transplantation in a yucatan miniature swine model

Douglas M. Smith, Judith A. Stribley, Robert P. Lieberman, John G. Sharp

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A large animal model is needed to evaluate new apheresis technologies. These technologies include novel methods of harvesting the blood mononuclear cell population which contains the hematopoietic stem cells needed to restore hematopoiesis in recipients of hematopoietically lethal therapy and the use of cytokines to provide a safe and predictable method of manipulating these circulating hematopoietic stem cells. We describe the methods used to collect mononuclear cells by leukapheresis from Yucatan miniature swine. These animals are of sufficient size to tolerate the procedures and have many physiologic and hematologic similarities to man. They are of good temperament and are easily trained. Long‐term venous access was obtained using single lumen silicone rubber catheters placed in the inferior vena cava. The animals were apheresed while fully awake using a Haemonetics Model V50 machine and a modified lymphocyte collection protocol. The procedure was highly efficient for the collection of mononuclear cells and a 10 pass procedure yielded a product which contained 19.7 × 109 mononuclear cells, 10.7 × 109 granulocytes, and 17 ml of erythrocytes in a volume of approximately 100 ml. This product can be cryopreserved and used for subsequent transplantation. The content of four apheresis procedures provides hematopoietic reconstitution of lethally irradiated swine on a time scale equivalent to transplantation of optimal numbers of bone marrow cells. © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalJournal of Clinical Apheresis
Volume7
Issue number2
DOIs
StatePublished - 1992

Keywords

  • apheresis
  • hematopoietic stem cells
  • inferior vena cava catheters
  • total body irradiation

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Collection of blood mononuclear cells by leukapheresis for transplantation in a yucatan miniature swine model'. Together they form a unique fingerprint.

  • Cite this