Colonoscopy-based intramucosal transplantation of cancer cells for mouse modeling of colon cancer and lung metastasis

Rizwan Ahmad, Balawant Kumar, Raju Lama Tamang, Wei Xu, Geoffrey A. Talmon, Aaron M. Mohs, Punita Dhawan, Amar B. Singh

Research output: Contribution to journalArticlepeer-review

Abstract

The conventional orthotopic/xenograft models or genetically engineered murine models of colon cancer (CRC) are limited in their scope for a true understanding of tumor growth, progression and eventual metastasis in its natural microenvironment. In the currently used murine models of CRC metastasis, the metastasis occurs primarily in the liver, though lung metastasis accounts for a significant proportion of CRC metastasis. There is an urgent need for a murine model of CRC, which not only allows tumor progression in the colonic mucosa but also metastasis of the lung. The authors describe a minimally invasive murine model of colon cancer progression that may be ideal for a wide range of applications, including evaluating gene function, microenvironment, cancer metastasis and therapeutic translational research.

Original languageEnglish (US)
Article number0172
JournalBioTechniques
Volume71
Issue number3
DOIs
StatePublished - Sep 2021

Keywords

  • Colon cancer
  • Colonoscopy
  • Lung metastasis
  • Metastasis
  • Xenograft

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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