Combining mass spectrometry with database interrogation strategies in proteomics

Adam J. Liska, Andrej Shevchenko

Research output: Contribution to journalReview articlepeer-review


Protein identification by mass spectrometry (MS) and sequence-database searching was established in 1993; since then, the proteomics community has witnessed a proliferation of analytical strategies for protein identification. Analytical strategies comprise three components: MS platforms; spectra-database sequence correlation methods; and, sequence databases. Multiple strategies are now applied simultaneously to increase sensitivity, throughput, and reliability of the characterization of proteomes. Now, by assessing the complexity of the interplay of MS, bioinformatics and sequence databases, we can begin to predict future approaches and challenges in the development of proteomics.

Original languageEnglish (US)
Pages (from-to)291-298
Number of pages8
JournalTrAC - Trends in Analytical Chemistry
Issue number5
StatePublished - May 1 2003
Externally publishedYes


  • Bioinformatics
  • Databases
  • Mass spectrometry
  • MS/MS
  • Proteomics
  • Spectra interpretation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy


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