Comparative kinetic modeling of growth and molecular hydrogen overproduction by engineered strains of Thermotoga maritima

Raghuveer Singh, Rahul Tevatia, Derrick White, Yaşar Demirel, Paul Blum

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Thermotoga maritima is an anaerobic hyperthermophilic bacterium known for its high amounts of hydrogen (H 2 ) production. In the current study, the kinetic modeling was applied on the engineered strains of T. maritima that surpassed the natural H 2 production limit. The study generated a kinetic model explaining H 2 overproduction and predicted a continuous fermentation system. A Leudking-Piret equation-based model predicted that H 2 production by Tma200 (0.217 mol-H 2 g −1 -biomass) and Tma100 (0.147 mol-H 2 g −1 -biomass) were higher than wild type (0.096 mol-H 2 g −1 -biomass) with reduced rates of maltose utilization. Sensitivity analysis confirmed satisfactory fitting of the experimental data. The slow growth rates of Tma200 (0.550 h −1 ) and Tma100 (0.495 h −1 ) are compared with the wild type (0.663 h −1 ). A higher maintenance energy along with growth and non-growth H 2 coefficients corroborate the higher H 2 productivity of the engineered strains. The modeled data established a continuous fermentation system for the sustainable H 2 production.

Original languageEnglish (US)
Pages (from-to)7125-7136
Number of pages12
JournalInternational Journal of Hydrogen Energy
Volume44
Issue number14
DOIs
StatePublished - Mar 15 2019

Keywords

  • Bio hydrogen beyond Thauer limit
  • Biofuel
  • Continuous stirred tank reactor
  • Dark fermentation
  • Kinetic modeling
  • Natural gas phase out

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Fingerprint Dive into the research topics of 'Comparative kinetic modeling of growth and molecular hydrogen overproduction by engineered strains of Thermotoga maritima'. Together they form a unique fingerprint.

  • Cite this