Comparative kinetic modeling of growth and molecular hydrogen overproduction by engineered strains of Thermotoga maritima

Raghuveer Singh, Rahul Tevatia, Derrick White, Yaşar Demirel, Paul Blum

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Thermotoga maritima is an anaerobic hyperthermophilic bacterium known for its high amounts of hydrogen (H2) production. In the current study, the kinetic modeling was applied on the engineered strains of T. maritima that surpassed the natural H2 production limit. The study generated a kinetic model explaining H2 overproduction and predicted a continuous fermentation system. A Leudking-Piret equation-based model predicted that H2 production by Tma200 (0.217 mol-H2 g−1-biomass) and Tma100 (0.147 mol-H2 g−1-biomass) were higher than wild type (0.096 mol-H2 g−1 -biomass) with reduced rates of maltose utilization. Sensitivity analysis confirmed satisfactory fitting of the experimental data. The slow growth rates of Tma200 (0.550 h−1) and Tma100 (0.495 h−1) are compared with the wild type (0.663 h−1). A higher maintenance energy along with growth and non-growth H2 coefficients corroborate the higher H2 productivity of the engineered strains. The modeled data established a continuous fermentation system for the sustainable H2 production.

Original languageEnglish (US)
Pages (from-to)7125-7136
Number of pages12
JournalInternational Journal of Hydrogen Energy
Volume44
Issue number14
DOIs
StatePublished - Mar 15 2019

Keywords

  • Bio hydrogen beyond Thauer limit
  • Biofuel
  • Continuous stirred tank reactor
  • Dark fermentation
  • Kinetic modeling
  • Natural gas phase out

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

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