TY - JOUR
T1 - Comparative manufacture and cell-based delivery of antiretroviral nanoformulations
AU - Balkundi, Shantanu
AU - Nowacek, Ari S.
AU - Veerubhotla, Ram S.
AU - Chen, Han
AU - Martinez-Skinner, Andrea
AU - Roy, Upal
AU - Mosley, R. Lee
AU - Kanmogne, Georgette
AU - Liu, Xinming
AU - Kabanov, Alexander V.
AU - Bronich, Tatiana
AU - McMillan, Joellyn
AU - Gendelman, Howard E.
N1 - Publisher Copyright:
© 2011 Balkundi et al, pwhich permits unrestric.
PY - 2011
Y1 - 2011
N2 - Nanoformulations of crystalline indinavir, ritonavir, atazanavir, and efavirenz were manufactured by wet milling, homogenization or sonication with a variety of excipients. The chemical, biological, immune, virological, and toxicological properties of these formulations were compared using an established monocyte-derived macrophage scoring indicator system. Measurements of drug uptake, retention, release, and antiretroviral activity demonstrated differences amongst preparation methods. Interestingly, for drug cell targeting and antiretroviral responses the most significant difference among the particles was the drug itself. We posit that the choice of drug and formulation composition may ultimately affect clinical utility.
AB - Nanoformulations of crystalline indinavir, ritonavir, atazanavir, and efavirenz were manufactured by wet milling, homogenization or sonication with a variety of excipients. The chemical, biological, immune, virological, and toxicological properties of these formulations were compared using an established monocyte-derived macrophage scoring indicator system. Measurements of drug uptake, retention, release, and antiretroviral activity demonstrated differences amongst preparation methods. Interestingly, for drug cell targeting and antiretroviral responses the most significant difference among the particles was the drug itself. We posit that the choice of drug and formulation composition may ultimately affect clinical utility.
KW - Human immunodeficiency virus type one
KW - Manufacturing techniques
KW - Monocyte-derived macrophage
KW - Nanoformulated antiretroviral therapy
KW - Nanotoxicology
UR - http://www.scopus.com/inward/record.url?scp=84864762785&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864762785&partnerID=8YFLogxK
U2 - 10.2147/IJN.S27830
DO - 10.2147/IJN.S27830
M3 - Article
C2 - 22267924
AN - SCOPUS:84864762785
SN - 1176-9114
VL - 6
SP - 3393
EP - 3404
JO - International journal of nanomedicine
JF - International journal of nanomedicine
ER -