Comparative studies of bacteria with an atomic force microscopy operating in different modes

A. V. Bolshakova; O. I. Kiselyova; A. S. Filonov; O. Yu Frolova; Y. L. Lyubchenko; I. V. Yaminsky

doi:10.1016/S0304-3991(00)00075-9

Comparative studies of bacteria with an atomic force microscopy operating in different modes

A. V. Bolshakova, O. I. Kiselyova, A. S. Filonov, O. Yu Frolova, Y. L. Lyubchenko, I. V. Yaminsky

Research output: Contribution to journal › Article › peer-review

144 Scopus citations

Abstract

Escherichia coli bacterial cells of two strains JM109 and K12 J62 were imaged with atomic force microscopy (AFM) in different environmental conditions. The AFM results show that the two strains have considerable difference in the surface morphology. At the same time after rehydration both strains show the loss of the topographic features and increase in lateral and vertical dimensions. Results obtained in different AFM modes (contact, tapping, MAC) were compared. Imaging in culture medium was applied for direct observation of the surface degradation effect of lysozyme. The treatment of the cells with the enzyme in the culture medium lead to the loss of surface rigidity and eventually to dramatic changes of the bacteria shape.

Original language	English (US)
Pages (from-to)	121-128
Number of pages	8
Journal	Ultramicroscopy
Volume	86
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3991(00)00075-9
State	Published - 2001
Externally published	Yes

Keywords

Atomic force microscopy
Bacteria
Lysozyme
Surface morphology

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Instrumentation

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10.1016/S0304-3991(00)00075-9

Cite this

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title = "Comparative studies of bacteria with an atomic force microscopy operating in different modes",

abstract = "Escherichia coli bacterial cells of two strains JM109 and K12 J62 were imaged with atomic force microscopy (AFM) in different environmental conditions. The AFM results show that the two strains have considerable difference in the surface morphology. At the same time after rehydration both strains show the loss of the topographic features and increase in lateral and vertical dimensions. Results obtained in different AFM modes (contact, tapping, MAC) were compared. Imaging in culture medium was applied for direct observation of the surface degradation effect of lysozyme. The treatment of the cells with the enzyme in the culture medium lead to the loss of surface rigidity and eventually to dramatic changes of the bacteria shape.",

keywords = "Atomic force microscopy, Bacteria, Lysozyme, Surface morphology",

author = "Bolshakova, {A. V.} and Kiselyova, {O. I.} and Filonov, {A. S.} and Frolova, {O. Yu} and Lyubchenko, {Y. L.} and Yaminsky, {I. V.}",

note = "Funding Information: The authors thank the Keck BioImaging laboratory (Arizona State University) for the use of their AFM equipment and Dr. J. Zhu (Molecular Imaging Corp., Phoenix AZ) for the help in imaging in liquid. This work was supported in part by the Russian Foundation for Basic Research (Grant 00-07-90016), Russian Ministry of Science and Technology, Program “Surface Atomic Structures (Grant No. 1.11.99), NATO Science Program (Linkage Grant LST.CLG.975.161) and the NIH grant GM 54991. ",

year = "2001",

doi = "10.1016/S0304-3991(00)00075-9",

language = "English (US)",

volume = "86",

pages = "121--128",

journal = "Ultramicroscopy",

issn = "0304-3991",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Comparative studies of bacteria with an atomic force microscopy operating in different modes

AU - Bolshakova, A. V.

AU - Kiselyova, O. I.

AU - Filonov, A. S.

AU - Frolova, O. Yu

AU - Lyubchenko, Y. L.

AU - Yaminsky, I. V.

N1 - Funding Information: The authors thank the Keck BioImaging laboratory (Arizona State University) for the use of their AFM equipment and Dr. J. Zhu (Molecular Imaging Corp., Phoenix AZ) for the help in imaging in liquid. This work was supported in part by the Russian Foundation for Basic Research (Grant 00-07-90016), Russian Ministry of Science and Technology, Program “Surface Atomic Structures (Grant No. 1.11.99), NATO Science Program (Linkage Grant LST.CLG.975.161) and the NIH grant GM 54991.

PY - 2001

Y1 - 2001

N2 - Escherichia coli bacterial cells of two strains JM109 and K12 J62 were imaged with atomic force microscopy (AFM) in different environmental conditions. The AFM results show that the two strains have considerable difference in the surface morphology. At the same time after rehydration both strains show the loss of the topographic features and increase in lateral and vertical dimensions. Results obtained in different AFM modes (contact, tapping, MAC) were compared. Imaging in culture medium was applied for direct observation of the surface degradation effect of lysozyme. The treatment of the cells with the enzyme in the culture medium lead to the loss of surface rigidity and eventually to dramatic changes of the bacteria shape.

AB - Escherichia coli bacterial cells of two strains JM109 and K12 J62 were imaged with atomic force microscopy (AFM) in different environmental conditions. The AFM results show that the two strains have considerable difference in the surface morphology. At the same time after rehydration both strains show the loss of the topographic features and increase in lateral and vertical dimensions. Results obtained in different AFM modes (contact, tapping, MAC) were compared. Imaging in culture medium was applied for direct observation of the surface degradation effect of lysozyme. The treatment of the cells with the enzyme in the culture medium lead to the loss of surface rigidity and eventually to dramatic changes of the bacteria shape.

KW - Atomic force microscopy

KW - Bacteria

KW - Lysozyme

KW - Surface morphology

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U2 - 10.1016/S0304-3991(00)00075-9

DO - 10.1016/S0304-3991(00)00075-9

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AN - SCOPUS:0035136391

SN - 0304-3991

VL - 86

SP - 121

EP - 128

JO - Ultramicroscopy

JF - Ultramicroscopy

IS - 1-2

ER -

Comparative studies of bacteria with an atomic force microscopy operating in different modes

Abstract

Keywords

ASJC Scopus subject areas

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