Comparative studies on expression of tumor-associated antigens in human and induced pancreatic cancer in syrian hamsters

Hiroshi Egami, Yoshiyuki Takiyama, William G. Chaney, Martin Cano, Hideki Fujii, Tsutoma Tomioka, Richard Metzgar, Parviz M. Pour

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


The expression of blood-group-related antigens (BGRAs) in experimental primary pancreatic cancer induced by N-nitrosobis(2-oxopropyl)amine (BOP) treatment of Syrian hamsters and homologous subcutaneous transplants of this primary cancer in the cell line, PC-1, established from the primary cancer and intrapancreatic transplanted PC-1 cells were studied by histochemical and biochemical methods. Human primary pancreatic cancer; the human pancreatic cancer cell line, HPAF; and its subclones, CDU and CD18, also were studied on a comparative basis. Histochemical analysis of BGRAs demonstrated that A, B, H, Leb, Lex Ley, and T antigen were expressed both in vivo and in vitro in hamster and human materials in similar patterns. However, Lea, CA 19-9 and sialylated Tn antigens were not found in hamster-derived tissues. SDS-PAGE and Western blotting procedures using anti-A antigen revealed similar major bands in the membrane fractions of both human and hamster pancreatic cells between 97 and 200 kdalton. Among other human pancreatic cancerassociated antigens, TAG-72, CA 125, and 17-1A were detected immunohistochemically in the hamster tumors both in vivo and in vitro, in a pattern similar to that seen in human pancreatic cancer. Tumor antigen DU-PAN-2, associated with human pancreatic cancer, was found infrequently in hamster pancreatic cancer specimens. These results indicate that the experimental hamster pancreatic cancer model provides a unique tool for investigating antigenicity of pancreatic cancer, particularly in relation to diagnosis and therapy.

Original languageEnglish (US)
Pages (from-to)91-100
Number of pages10
JournalInternational Journal of Pancreatology
Issue number1-3
StatePublished - Aug 1990


  • Tumor immunology
  • blood group antigens
  • hamster
  • human
  • pancreatic cancer

ASJC Scopus subject areas

  • Oncology
  • Endocrinology
  • Gastroenterology


Dive into the research topics of 'Comparative studies on expression of tumor-associated antigens in human and induced pancreatic cancer in syrian hamsters'. Together they form a unique fingerprint.

Cite this