Comparison of amino acid sequences at the amino acid 130-160 region of VP1 polypeptide of Indian field isolates of foot-and-mouth disease virus serotype Asia 1

A. Sanyal, C. B. Gurumurthy, R. Venkataramanan, D. Hemadri, C. Tosh

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The nucleotide and deduced amino acid sequences in the amino acid (aa) 130-160 region of VP1 polypeptide of 65 field isolates of foot and mouth disease virus (FMDV) serotype Asia 1 were determined and the consensus sequences were deduced. Comparison of amino acid sequences revealed conservation of NGK (130-132), TYG (134-136), RGD (142-144), and LPTSF (156-160) motifs and aa 148 (L) while Variation was observed at the rest of the region (variability index (VI) of 2.06 to 16.85). Synonymous and non-synonymous mutations at the nucleotide level were well correlated with those of the corresponding amino acids. Comparison of the aa 130-160 sequence of Asia 1 serotype with those of other serotypes of FMDV revealed conservation of aa 135, 148-149, 157 and 160. Amino acids 133-138 and 148-154 were unique for Asia 1 serotype and are presumably responsible for its distinct antigenic nature. The present study revealed that the FMDV isolates of serotype Asia 1 causing outbreaks in India are very much heterogeneous in the aa 130-160 region of VP1.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalActa Virologica
Volume44
Issue number2
StatePublished - 2000

Keywords

  • Amino acid sequence
  • FMD virus
  • Indian isolates
  • Serotype Asia 1
  • VP1
  • Variability index

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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