TY - JOUR
T1 - Comparison of Disposition Values Obtained by Two Assay Methods for Quinidine Gluconate in Patients with Ventricular Tachycardia
AU - Ackerman, Bruce H.
AU - Olsen, Keith M.
AU - Kennedy, Eleanor E.
AU - Taylor, E. Howard
AU - Chen, Bai H.
AU - Jordan, Drexel
AU - Ackerman, Deborah J.
PY - 1989
Y1 - 1989
N2 - Eight patients with previously untreated ventricular tachycardia, age 48.54 ± 28.02 years (mean ± SD), were enrolled in a protocol evaluating the disposition of quinidine gluconate as determined by two assay methods. Patients received two infusions of 5 mg/kg over 30 minutes separated by 20–30 (24.9 ± 4.0) minutes of electrophysiologic testing. Blood samples were obtained at 0.17 hours and just prior to the second infusion, and then at 0.17, 0.25, 0.33, 1.0, 6.0, 12.0, and 24.0 hours after the second infusion. Paired serum samples were assayed for quinidine concentrations by fluorescence polarization immunoassay and high‐performance liquid chromatography. The two assays compared well, with a linear regression equation of Y = 0.927X + 0.247 with a correlation coefficient of 0.985. With the exception of the ß elimination rate constant and ß distribution volume, t test comparison of disposition values demonstrated no significant difference. Differences in the estimates of the ß elimination rate constant reflected differences in the two methods and indicated that even though both assays were comparable, subtle differences in specificity could be reflected in significant differences in this variable. 1989 Pharmacotherapy Publications Inc.
AB - Eight patients with previously untreated ventricular tachycardia, age 48.54 ± 28.02 years (mean ± SD), were enrolled in a protocol evaluating the disposition of quinidine gluconate as determined by two assay methods. Patients received two infusions of 5 mg/kg over 30 minutes separated by 20–30 (24.9 ± 4.0) minutes of electrophysiologic testing. Blood samples were obtained at 0.17 hours and just prior to the second infusion, and then at 0.17, 0.25, 0.33, 1.0, 6.0, 12.0, and 24.0 hours after the second infusion. Paired serum samples were assayed for quinidine concentrations by fluorescence polarization immunoassay and high‐performance liquid chromatography. The two assays compared well, with a linear regression equation of Y = 0.927X + 0.247 with a correlation coefficient of 0.985. With the exception of the ß elimination rate constant and ß distribution volume, t test comparison of disposition values demonstrated no significant difference. Differences in the estimates of the ß elimination rate constant reflected differences in the two methods and indicated that even though both assays were comparable, subtle differences in specificity could be reflected in significant differences in this variable. 1989 Pharmacotherapy Publications Inc.
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U2 - 10.1002/j.1875-9114.1989.tb04129.x
DO - 10.1002/j.1875-9114.1989.tb04129.x
M3 - Article
C2 - 2771807
AN - SCOPUS:0024462732
SN - 0277-0008
VL - 9
SP - 220
EP - 225
JO - Pharmacotherapy
JF - Pharmacotherapy
IS - 4
ER -