This study was designed to evaluate the performance of EnviroGard immunoassay kits for the field analysis of pesticides in surface waters in Malaysia. The primary objective was to determine possible effects of the surface water matrix or environmental conditions on the sensitivity or reliability of the EnviroGard immunoassay kits. The kits used in this study were: alachlor, triazines, diazinon, cyclodienes, paraquat, and 2,4-diclorophenoxyacetic acid. Field work consisted of enzyme immunoassay (EIA) of surface water samples using the EnviroGard plate kits in conjunction with a Millipore Microwell strip reader. Split samples of surface waters, blanks, and spikes were processed in the field using solid phase extraction (SPE) disks (C8 or C18) and transported to University of Iowa, where extracted pesticides were eluted and analyzed using high pressure liquid chromatography (HPLC) and gas chromatography / mass spectrometry (GC/MS). HPLC and GC/MS data were corrected for pesticide recovery and stability on the C8 or C18 during transit. Good agreement was obtained between the field EIA and laboratory HPLC or GC/MS analysis of field spiked samples for all kits with the exception of paraquat. The results of this study indicate that the EnviroGard enzyme immunoassay is an efficient and reliable field testing tool for pesticides in water.
|Original language||English (US)|
|Number of pages||11|
|Journal||ACS Symposium Series|
|State||Published - 1996|
ASJC Scopus subject areas
- Chemical Engineering(all)