Comparison of the antiproliferative effects of transforming growth factor-β, N,N-dimethylformamide and retinoic acid on a human colon carcinoma cell line

Naseema M. Hoosein; Diane E. Brattain; Mary K. McKnight; Karla E. Childress; Subhas Chakrabarty; Michael G. Brattain

doi:10.1016/0304-3835(88)90014-6

Comparison of the antiproliferative effects of transforming growth factor-β, N,N-dimethylformamide and retinoic acid on a human colon carcinoma cell line

Naseema M. Hoosein, Diane E. Brattain, Mary K. McKnight, Karla E. Childress, Subhas Chakrabarty, Michael G. Brattain

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

In this study we have compared the anti-proliferative effects of transforming growth factor-β (TGF-β), N,N-dimethylformamide (DMF) and retinoic acid (RA) on a moderately-differentiated colon carcinoma cell line (JVC). TGF-β, DMF and RA inhibited the anchorage-independent growth and induced morphological changes in JVC cells. EC₅₀ values of 40 pM TGF-β, 0.5% DMF and 5 nM RA were obtained for growth inhibition. In addition all three agents enhanced cellular fibronectin levels in a time- and dose-dependent manner. Inhibition of cell proliferation as well as fibronectin induction by all three agents were reversible. Combinations of any two agents at suboptimal doses, added simultaneously to JVC cells gave additive inhibitory response on growth. These data indicate that the effects of TGF-β in this colon carcinoma cell line are similar to those of the two differentiation promoting agents DMF and RA.

Original language	English (US)
Pages (from-to)	219-232
Number of pages	14
Journal	Cancer Letters
Volume	40
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(88)90014-6
State	Published - Jun 15 1988
Externally published	Yes

Keywords

Colon carcinoma cell line
Dimethylformamide
Fibronectin production
Retinoic acid
Transforming growth factor β

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(88)90014-6

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@article{9cabde811ab64b549d7444a5e927a7de,

title = "Comparison of the antiproliferative effects of transforming growth factor-β, N,N-dimethylformamide and retinoic acid on a human colon carcinoma cell line",

abstract = "In this study we have compared the anti-proliferative effects of transforming growth factor-β (TGF-β), N,N-dimethylformamide (DMF) and retinoic acid (RA) on a moderately-differentiated colon carcinoma cell line (JVC). TGF-β, DMF and RA inhibited the anchorage-independent growth and induced morphological changes in JVC cells. EC50 values of 40 pM TGF-β, 0.5% DMF and 5 nM RA were obtained for growth inhibition. In addition all three agents enhanced cellular fibronectin levels in a time- and dose-dependent manner. Inhibition of cell proliferation as well as fibronectin induction by all three agents were reversible. Combinations of any two agents at suboptimal doses, added simultaneously to JVC cells gave additive inhibitory response on growth. These data indicate that the effects of TGF-β in this colon carcinoma cell line are similar to those of the two differentiation promoting agents DMF and RA.",

keywords = "Colon carcinoma cell line, Dimethylformamide, Fibronectin production, Retinoic acid, Transforming growth factor β",

author = "Hoosein, {Naseema M.} and Brattain, {Diane E.} and McKnight, {Mary K.} and Childress, {Karla E.} and Subhas Chakrabarty and Brattain, {Michael G.}",

note = "Funding Information: We thank Andrew A. Tobon for processing cells for immunofluorescence and Ms. Gail Rudder and Arlene Cain for typing this manuscript. This work was supported by Grants CA38173 and CA34432 from the National Institutes of Health.",

year = "1988",

month = jun,

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doi = "10.1016/0304-3835(88)90014-6",

language = "English (US)",

volume = "40",

pages = "219--232",

journal = "Cancer Letters",

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T1 - Comparison of the antiproliferative effects of transforming growth factor-β, N,N-dimethylformamide and retinoic acid on a human colon carcinoma cell line

AU - Hoosein, Naseema M.

AU - Brattain, Diane E.

AU - McKnight, Mary K.

AU - Childress, Karla E.

AU - Chakrabarty, Subhas

AU - Brattain, Michael G.

N1 - Funding Information: We thank Andrew A. Tobon for processing cells for immunofluorescence and Ms. Gail Rudder and Arlene Cain for typing this manuscript. This work was supported by Grants CA38173 and CA34432 from the National Institutes of Health.

PY - 1988/6/15

Y1 - 1988/6/15

N2 - In this study we have compared the anti-proliferative effects of transforming growth factor-β (TGF-β), N,N-dimethylformamide (DMF) and retinoic acid (RA) on a moderately-differentiated colon carcinoma cell line (JVC). TGF-β, DMF and RA inhibited the anchorage-independent growth and induced morphological changes in JVC cells. EC50 values of 40 pM TGF-β, 0.5% DMF and 5 nM RA were obtained for growth inhibition. In addition all three agents enhanced cellular fibronectin levels in a time- and dose-dependent manner. Inhibition of cell proliferation as well as fibronectin induction by all three agents were reversible. Combinations of any two agents at suboptimal doses, added simultaneously to JVC cells gave additive inhibitory response on growth. These data indicate that the effects of TGF-β in this colon carcinoma cell line are similar to those of the two differentiation promoting agents DMF and RA.

AB - In this study we have compared the anti-proliferative effects of transforming growth factor-β (TGF-β), N,N-dimethylformamide (DMF) and retinoic acid (RA) on a moderately-differentiated colon carcinoma cell line (JVC). TGF-β, DMF and RA inhibited the anchorage-independent growth and induced morphological changes in JVC cells. EC50 values of 40 pM TGF-β, 0.5% DMF and 5 nM RA were obtained for growth inhibition. In addition all three agents enhanced cellular fibronectin levels in a time- and dose-dependent manner. Inhibition of cell proliferation as well as fibronectin induction by all three agents were reversible. Combinations of any two agents at suboptimal doses, added simultaneously to JVC cells gave additive inhibitory response on growth. These data indicate that the effects of TGF-β in this colon carcinoma cell line are similar to those of the two differentiation promoting agents DMF and RA.

KW - Colon carcinoma cell line

KW - Dimethylformamide

KW - Fibronectin production

KW - Retinoic acid

KW - Transforming growth factor β

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Comparison of the antiproliferative effects of transforming growth factor-β, N,N-dimethylformamide and retinoic acid on a human colon carcinoma cell line

Abstract

Keywords

ASJC Scopus subject areas

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