TY - JOUR
T1 - Comparison of the effects of transforming growth factor β, N,N-dimethylformamide, and retinoic acid on transformed and nontransformed fibroblasts
AU - Hoosein, Naseema M.
AU - Brattain, Diane E.
AU - McKnight, Mary K.
AU - Brattain, Michael G.
PY - 1988/3
Y1 - 1988/3
N2 - In order to compare the effects of transforming growth factor (TGFβ) with those of the differentiation promoters N,N-dimethylformamide (DMF) and retinoic acid (RA), the antiproliferative and fibronectin-inducing activities of the three agents were examined. AKR-2B mouse embryo fibroblasts and their chemically transformed counterpart AKR-MCA cells were used as the model system. Growth in monolayer culture of both cell lines was inhibited by TGFβ (EC50 ~1 ng/ml), DMF (EC50 ~0.5%), and RA (EC50 ~1 μM) in a concentration-dependent manner. Time-dependent elevation in fibronectin expression was also observed with all three agents. The EC50 for growth inhibition of both cell lines by TGFβ agreed well with that obtained for stimulation of fibronectin synthesis. A 3-h exposure to TGFβ is sufficient to obtain the maximal fibronectin level observed at 48 h in AKR-2B cells but not in AKR-MCA cells. Our results indicate that in this system the effects of TGFβ are similar to those of the chemical differentiation inducers DMF and RA. Furthermore, our data also suggest that the TGFβ signal may be processed differently by nontransformed and transformed fibroblasts.
AB - In order to compare the effects of transforming growth factor (TGFβ) with those of the differentiation promoters N,N-dimethylformamide (DMF) and retinoic acid (RA), the antiproliferative and fibronectin-inducing activities of the three agents were examined. AKR-2B mouse embryo fibroblasts and their chemically transformed counterpart AKR-MCA cells were used as the model system. Growth in monolayer culture of both cell lines was inhibited by TGFβ (EC50 ~1 ng/ml), DMF (EC50 ~0.5%), and RA (EC50 ~1 μM) in a concentration-dependent manner. Time-dependent elevation in fibronectin expression was also observed with all three agents. The EC50 for growth inhibition of both cell lines by TGFβ agreed well with that obtained for stimulation of fibronectin synthesis. A 3-h exposure to TGFβ is sufficient to obtain the maximal fibronectin level observed at 48 h in AKR-2B cells but not in AKR-MCA cells. Our results indicate that in this system the effects of TGFβ are similar to those of the chemical differentiation inducers DMF and RA. Furthermore, our data also suggest that the TGFβ signal may be processed differently by nontransformed and transformed fibroblasts.
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U2 - 10.1016/0014-4827(88)90260-1
DO - 10.1016/0014-4827(88)90260-1
M3 - Article
C2 - 3162213
AN - SCOPUS:0023924120
SN - 0014-4827
VL - 175
SP - 125
EP - 135
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -