Comparison of the gen-probe PACE2(TM) system, direct fluorescent-antibody, and cell culture for detecting Chlamydia trachomatis in cervical specimens

P. C. Iwen, T. M.H. Blair, G. L. Woods

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

A chemiluminescent-labeled DNA probe (Gen-Probe PACE(TM) 2 System) for detection of Chlamydia trachomatis in endocervical specimens was evaluated. For each specimen, tissue cell culture, direct immunofluorecent staining (DFA), and DNA probe assay were performed. Thirty of 318 (9.4%) specimens were positive for C. trachomatis. The sensitivities, specificities, and positive and negative predictive values of the DNA probe compared with cell culture were 93%, 98%, 85%, and 99%, respectively, and for DFA these same values were 81%, 99%, 83%, and 99%, respectively. The Gen-Probe PACE 2 System is a reliable method for the rapid detection of endocervical chlamydial infection.

Original languageEnglish (US)
Pages (from-to)578-582
Number of pages5
JournalAmerican journal of clinical pathology
Volume95
Issue number4
DOIs
StatePublished - 1991

Keywords

  • Chlamydia trachomatis
  • Clinical diagnosis
  • DNA probe
  • Laboratory diagnosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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