Comparison of the gen-probe PACE2(TM) system, direct fluorescent-antibody, and cell culture for detecting Chlamydia trachomatis in cervical specimens

P. C. Iwen; T. M.H. Blair; G. L. Woods

doi:10.1093/ajcp/95.4.578

Comparison of the gen-probe PACE2(TM) system, direct fluorescent-antibody, and cell culture for detecting Chlamydia trachomatis in cervical specimens

P. C. Iwen, T. M.H. Blair, G. L. Woods

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A chemiluminescent-labeled DNA probe (Gen-Probe PACE(TM) 2 System) for detection of Chlamydia trachomatis in endocervical specimens was evaluated. For each specimen, tissue cell culture, direct immunofluorecent staining (DFA), and DNA probe assay were performed. Thirty of 318 (9.4%) specimens were positive for C. trachomatis. The sensitivities, specificities, and positive and negative predictive values of the DNA probe compared with cell culture were 93%, 98%, 85%, and 99%, respectively, and for DFA these same values were 81%, 99%, 83%, and 99%, respectively. The Gen-Probe PACE 2 System is a reliable method for the rapid detection of endocervical chlamydial infection.

Original language	English (US)
Pages (from-to)	578-582
Number of pages	5
Journal	American journal of clinical pathology
Volume	95
Issue number	4
DOIs	https://doi.org/10.1093/ajcp/95.4.578
State	Published - 1991

Keywords

Chlamydia trachomatis
Clinical diagnosis
DNA probe
Laboratory diagnosis

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1093/ajcp/95.4.578

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title = "Comparison of the gen-probe PACE2(TM) system, direct fluorescent-antibody, and cell culture for detecting Chlamydia trachomatis in cervical specimens",

abstract = "A chemiluminescent-labeled DNA probe (Gen-Probe PACE(TM) 2 System) for detection of Chlamydia trachomatis in endocervical specimens was evaluated. For each specimen, tissue cell culture, direct immunofluorecent staining (DFA), and DNA probe assay were performed. Thirty of 318 (9.4%) specimens were positive for C. trachomatis. The sensitivities, specificities, and positive and negative predictive values of the DNA probe compared with cell culture were 93%, 98%, 85%, and 99%, respectively, and for DFA these same values were 81%, 99%, 83%, and 99%, respectively. The Gen-Probe PACE 2 System is a reliable method for the rapid detection of endocervical chlamydial infection.",

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author = "Iwen, {P. C.} and Blair, {T. M.H.} and Woods, {G. L.}",

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AU - Iwen, P. C.

AU - Blair, T. M.H.

AU - Woods, G. L.

PY - 1991

Y1 - 1991

N2 - A chemiluminescent-labeled DNA probe (Gen-Probe PACE(TM) 2 System) for detection of Chlamydia trachomatis in endocervical specimens was evaluated. For each specimen, tissue cell culture, direct immunofluorecent staining (DFA), and DNA probe assay were performed. Thirty of 318 (9.4%) specimens were positive for C. trachomatis. The sensitivities, specificities, and positive and negative predictive values of the DNA probe compared with cell culture were 93%, 98%, 85%, and 99%, respectively, and for DFA these same values were 81%, 99%, 83%, and 99%, respectively. The Gen-Probe PACE 2 System is a reliable method for the rapid detection of endocervical chlamydial infection.

AB - A chemiluminescent-labeled DNA probe (Gen-Probe PACE(TM) 2 System) for detection of Chlamydia trachomatis in endocervical specimens was evaluated. For each specimen, tissue cell culture, direct immunofluorecent staining (DFA), and DNA probe assay were performed. Thirty of 318 (9.4%) specimens were positive for C. trachomatis. The sensitivities, specificities, and positive and negative predictive values of the DNA probe compared with cell culture were 93%, 98%, 85%, and 99%, respectively, and for DFA these same values were 81%, 99%, 83%, and 99%, respectively. The Gen-Probe PACE 2 System is a reliable method for the rapid detection of endocervical chlamydial infection.

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Comparison of the gen-probe PACE2(TM) system, direct fluorescent-antibody, and cell culture for detecting Chlamydia trachomatis in cervical specimens

Abstract

Keywords

ASJC Scopus subject areas

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