Comparison of the in vivo and in vitro nuclear magnetic resonance detection of trifluoromethyl penicillin V in rats

G. Douglas Campbell, Subbaraya Ramaprasad, Keith M. Olsen, A. Francine Tryka, Richard A. Komoroski, Larry C. Blaszczak, Thomas R. Parr

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Fluorine nuclear magnetic resonance (NMR) spectroscopy and magnetic resonance imaging were examined as noninvasive methods for characterizing antibiotic disposition and pharmacokinetics in vivo. For determination of their utility, a 19F surface coil was constructed and an m‐(trifluoromethyl)‐containing penicillin V analogue (LY242072; 1) was synthesized. Various concentrations of 1 were injected intravenously into anesthetized rats whose urethras were occluded. The animals were placed on the surface coil, which was tuned to 19F, and then into a 4.7‐T, 33‐cm bore instrument, in which in vivo measurements of 1 were made on urine excreted into the bladder. At sacrifice, the urine was collected, and antibiotic leveis were determined in vitro by both HPLC and high‐resolution NMR. The limit of detection of 1 by NMR was 0.7 mg/mL of urine. When compared with standard in vitro quantitative methods using current technology, quantitation by in vivo surface coil NMR is not precise. Magnetic resonance imaging was used to image the bladder at a 35‐mm3 voxel resolution with datum collection times of ˜ 1 h. The 19F surface coil was used successfully to spectroscopically locate xenobiotic fluorine in the rat thorax. 19F NMR may offer an opportunity for the noninvasive in vivo detection of the distribution of various classes of therapeutic compounds.

Original languageEnglish (US)
Pages (from-to)48-51
Number of pages4
JournalJournal of Pharmaceutical Sciences
Issue number1
StatePublished - Jan 1993
Externally publishedYes

ASJC Scopus subject areas

  • Pharmaceutical Science


Dive into the research topics of 'Comparison of the in vivo and in vitro nuclear magnetic resonance detection of trifluoromethyl penicillin V in rats'. Together they form a unique fingerprint.

Cite this