Comparison of viral replication and IFN response in alpaca and bovine cells following bovine viral diarrhea virus infection

Holly C. Samson; Christina L. Topliff; Ruben O. Donis; Clayton L. Kelling

doi:10.1016/j.virol.2011.02.006

Comparison of viral replication and IFN response in alpaca and bovine cells following bovine viral diarrhea virus infection

Holly C. Samson, Christina L. Topliff, Ruben O. Donis, Clayton L. Kelling

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Alpacas develop diminished disease following bovine viral diarrhea virus (BVDV) infection compared to cattle. We hypothesized that alpaca and bovine cells have differential permissiveness and responses to BVDV infection. To characterize alpaca testicular (AT) and bovine turbinate (BT) cells BVDV infection permissiveness, viral replication and interferon (IFN) synthesis was evaluated. BVDV replicated 3-4 logs lower in AT cells with diminished antigen deposition compared to BT cells. BVDV infection inhibited IFN response in both AT and BT cells. Compared to BT cells, BVDV-infected AT cells had a 2-5 fold increase in IFN synthesis following dsRNA stimulation. The greater IFN response of AT cells compared to BT cells following poly I:C stimulation with or without ncp BVDV infection, may be the basis for the decreased BVDV permissiveness of AT cells and may contribute to the clinical differences following BVDV infection of alpacas and cattle.

Original language	English (US)
Pages (from-to)	111-117
Number of pages	7
Journal	Virology
Volume	413
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2011.02.006
State	Published - Apr 25 2011

Keywords

Alpaca
Bovine viral diarrhea virus
IFN
Permissiveness
Viral replication

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2011.02.006

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title = "Comparison of viral replication and IFN response in alpaca and bovine cells following bovine viral diarrhea virus infection",

abstract = "Alpacas develop diminished disease following bovine viral diarrhea virus (BVDV) infection compared to cattle. We hypothesized that alpaca and bovine cells have differential permissiveness and responses to BVDV infection. To characterize alpaca testicular (AT) and bovine turbinate (BT) cells BVDV infection permissiveness, viral replication and interferon (IFN) synthesis was evaluated. BVDV replicated 3-4 logs lower in AT cells with diminished antigen deposition compared to BT cells. BVDV infection inhibited IFN response in both AT and BT cells. Compared to BT cells, BVDV-infected AT cells had a 2-5 fold increase in IFN synthesis following dsRNA stimulation. The greater IFN response of AT cells compared to BT cells following poly I:C stimulation with or without ncp BVDV infection, may be the basis for the decreased BVDV permissiveness of AT cells and may contribute to the clinical differences following BVDV infection of alpacas and cattle.",

keywords = "Alpaca, Bovine viral diarrhea virus, IFN, Permissiveness, Viral replication",

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AU - Samson, Holly C.

AU - Topliff, Christina L.

AU - Donis, Ruben O.

AU - Kelling, Clayton L.

PY - 2011/4/25

Y1 - 2011/4/25

N2 - Alpacas develop diminished disease following bovine viral diarrhea virus (BVDV) infection compared to cattle. We hypothesized that alpaca and bovine cells have differential permissiveness and responses to BVDV infection. To characterize alpaca testicular (AT) and bovine turbinate (BT) cells BVDV infection permissiveness, viral replication and interferon (IFN) synthesis was evaluated. BVDV replicated 3-4 logs lower in AT cells with diminished antigen deposition compared to BT cells. BVDV infection inhibited IFN response in both AT and BT cells. Compared to BT cells, BVDV-infected AT cells had a 2-5 fold increase in IFN synthesis following dsRNA stimulation. The greater IFN response of AT cells compared to BT cells following poly I:C stimulation with or without ncp BVDV infection, may be the basis for the decreased BVDV permissiveness of AT cells and may contribute to the clinical differences following BVDV infection of alpacas and cattle.

AB - Alpacas develop diminished disease following bovine viral diarrhea virus (BVDV) infection compared to cattle. We hypothesized that alpaca and bovine cells have differential permissiveness and responses to BVDV infection. To characterize alpaca testicular (AT) and bovine turbinate (BT) cells BVDV infection permissiveness, viral replication and interferon (IFN) synthesis was evaluated. BVDV replicated 3-4 logs lower in AT cells with diminished antigen deposition compared to BT cells. BVDV infection inhibited IFN response in both AT and BT cells. Compared to BT cells, BVDV-infected AT cells had a 2-5 fold increase in IFN synthesis following dsRNA stimulation. The greater IFN response of AT cells compared to BT cells following poly I:C stimulation with or without ncp BVDV infection, may be the basis for the decreased BVDV permissiveness of AT cells and may contribute to the clinical differences following BVDV infection of alpacas and cattle.

KW - Alpaca

KW - Bovine viral diarrhea virus

KW - IFN

KW - Permissiveness

KW - Viral replication

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Comparison of viral replication and IFN response in alpaca and bovine cells following bovine viral diarrhea virus infection

Abstract

Keywords

ASJC Scopus subject areas

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