Compensation for the absence of the catalytically active half of DNA polymerase ϵ in yeast by positively selected mutations in CDC28

Elena I. Stepchenkova, Anna S. Zhuk, Jian Cui, Elena R. Tarakhovskaya, Stephanie R. Barbari, Polina V. Shcherbakova, Dmitrii E. Polev, Roman Fedorov, Eugenia Poliakov, Igor B. Rogozin, Artem G. Lada, Youri I. Pavlov

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Current eukaryotic replication models postulate that leading and lagging DNA strands are replicated predominantly by dedicated DNA polymerases. The catalytic subunit of the leading strand DNA polymerase ϵ, Pol2, consists of two halves made of two different ancestral B-family DNA polymerases. Counterintuitively, the catalytically active N-Terminal half is dispensable, while the inactive C-Terminal part is required for viability. Despite extensive studies of yeast Saccharomyces cerevisiae strains lacking the active N-Terminal half, it is still unclear how these strains survive and recover. We designed a robust method for constructing mutants with only the C-Terminal part of Pol2. Strains without the active polymerase part show severe growth defects, sensitivity to replication inhibitors, chromosomal instability, and elevated spontaneous mutagenesis. Intriguingly, the slow-growing mutant strains rapidly accumulate fast-growing clones. Analysis of genomic DNA sequences of these clones revealed that the adaptation to the loss of the catalytic N-Terminal part of Pol2 occurs by a positive selection of mutants with improved growth. Elevated mutation rates help generate sufficient numbers of these variants. Single nucleotide changes in the cell cycle-dependent kinase gene, CDC28, improve the growth of strains lacking the N-Terminal part of Pol2, and rescue their sensitivity to replication inhibitors and, in parallel, lower mutation rates. Our study predicts that changes in mammalian homologs of cyclin-dependent kinases may contribute to cellular responses to the leading strand polymerase defects.

Original languageEnglish (US)
Article numberiyab060
Issue number2
StatePublished - Jun 2021


  • CDC28
  • DNA polymerase ϵ
  • DNA replication
  • cyclin-dependent kinase
  • illegitimate mating
  • leading strand
  • mutation rates
  • yeast

ASJC Scopus subject areas

  • Medicine(all)


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