Competitive binding analysis of monoclonal antibodies reactive with porcine alveolar macrophages using anti-CD14 and anti-CD18

T. Kielian; D. S. McVey; W. C. Davis; Y. B. Kim; F. Blecha

doi:10.1016/0165-2427(94)90148-1

Competitive binding analysis of monoclonal antibodies reactive with porcine alveolar macrophages using anti-CD14 and anti-CD18

T. Kielian, D. S. McVey, W. C. Davis, Y. B. Kim, F. Blecha

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Four monoclonal antibodies (mAb) from the myeloid subset panel of the First International Swine CD Workshop (74-22-15, DH59B, PM16-6, and MUC21A) were analyzed using competitive inhibition studies with anti-human CD14 (My4) and anti-human/anti-porcine CD18 (MHM23) on porcine alveolar macrophages. Results suggested that none of the mAb tested recognized CD14 or CD18 on porcine alveolar macrophages. Additionally, the cross-reactivity of My4 with porcine alveolar macrophages was established.

Original language	English (US)
Pages (from-to)	273-278
Number of pages	6
Journal	Veterinary Immunology and Immunopathology
Volume	43
Issue number	1-3
DOIs	https://doi.org/10.1016/0165-2427(94)90148-1
State	Published - Oct 1994
Externally published	Yes

ASJC Scopus subject areas

Immunology
General Veterinary

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title = "Competitive binding analysis of monoclonal antibodies reactive with porcine alveolar macrophages using anti-CD14 and anti-CD18",

abstract = "Four monoclonal antibodies (mAb) from the myeloid subset panel of the First International Swine CD Workshop (74-22-15, DH59B, PM16-6, and MUC21A) were analyzed using competitive inhibition studies with anti-human CD14 (My4) and anti-human/anti-porcine CD18 (MHM23) on porcine alveolar macrophages. Results suggested that none of the mAb tested recognized CD14 or CD18 on porcine alveolar macrophages. Additionally, the cross-reactivity of My4 with porcine alveolar macrophages was established.",

author = "T. Kielian and McVey, {D. S.} and Davis, {W. C.} and Kim, {Y. B.} and F. Blecha",

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AU - McVey, D. S.

AU - Davis, W. C.

AU - Kim, Y. B.

AU - Blecha, F.

PY - 1994/10

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AB - Four monoclonal antibodies (mAb) from the myeloid subset panel of the First International Swine CD Workshop (74-22-15, DH59B, PM16-6, and MUC21A) were analyzed using competitive inhibition studies with anti-human CD14 (My4) and anti-human/anti-porcine CD18 (MHM23) on porcine alveolar macrophages. Results suggested that none of the mAb tested recognized CD14 or CD18 on porcine alveolar macrophages. Additionally, the cross-reactivity of My4 with porcine alveolar macrophages was established.

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Competitive binding analysis of monoclonal antibodies reactive with porcine alveolar macrophages using anti-CD14 and anti-CD18

Abstract

ASJC Scopus subject areas

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