Complement-dependent injury and protection in a murine model of acute dextran sulfate sodium-induced colitis

Jennifer Schepp-Berglind, Carl Atkinson, Michelle Elvington, Fei Qiao, Peter Mannon, Stephen Tomlinson

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Complement plays a key role in the pathophysiology of many inflammatory diseases, and in this study, we investigated the role of complement in the pathogenesis of inflammatory bowel disease. Compared to wild-type mice, mice deficient in C3 or factor B were protected from acute dextran sulfate sodium (DSS)-induced colitis. C1q/mannose-binding lectin (MBL) double-deficient mice, however, exhibited more severe colitis than wild-type mice. When mice were allowed to recover after DSS treatment, all C1q/MBL -/- mice died by day 2 of recovery period, and, surprisingly, all C3 -/- and factor B -/- mice died by day 5. Serum endotoxin levels were significantly increased in complement-deficient mice prior to death, particularly in C1q/MBL -/- mice, and antibiotic treatment prevented the lethal effect of DSS in all complement-deficient mice. In contrast to complement deficiency, targeted complement inhibition with either complement receptor 2 (CR2)-Crry (blocks all pathways at C3 activation) or CR2-factor H (blocks alternative pathway) was highly protective at treating established acute colitis. Endotoxin levels remained low in complement-inhibited mice, and complement inhibition also reduced inflammatory cytokines, leukocyte infiltration, and tissue injury while improving wound repair and mucosal healing. CR2-factor H provided more effective protection than CR2-Crry. Thus, complement has both pathogenic and protective roles in acute DSS-induced colitis, and whereas the alternative pathway appears to play a key role in tissue inflammation and injury, the classical/lectin pathway provides important protection in terms of host defense and wound repair. Targeted inhibition of the alternative pathway may represent a therapeutic modality for treating acute phases of inflammatory bowel disease.

Original languageEnglish (US)
Pages (from-to)6309-6318
Number of pages10
JournalJournal of Immunology
Issue number12
StatePublished - Jun 15 2012
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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