After systemic complement activation by cobra venom factor (CVF), which is known to produce a neutrophil dependent lung injury, the time course in the appearance of C5a in the serum of male Long Evans rats was measured over 30 minutes by ELISA. The effect of murine C5a on neutrophil chemotaxis and its inhibiton by a polyclonal rabbit anti rat antibody to C5a were also studied. High levels of C5a were reached as early as 1 minute after injection of CVF and remained elevated up to 30 minutes. The levels of C5a showed a dose response related to the dose of CVF injected. The chemotactic activity of C5a was inhibited by anti C5a whereas serum hemolytic complement activity (CH50 value) was not diminished by the antibody, suggesting that anti-C5a is not reactive with intact C5. The early appearance of C5a in the inflammatory process, its high chemotactic activity and its inhibition by anti C5a suggests that C5a plays a key role in acute inflammation that occurs as a result of complement activation.
|Original language||English (US)|
|State||Published - 1996|
ASJC Scopus subject areas
- Molecular Biology