Comprehensive bioimaging with fluorinated nanoparticles using breathable liquids

Michael E. Kurczy; Zheng Jiang Zhu; Julijana Ivanisevic; Adam M. Schuyler; Kush Lalwani; Antonio F. Santidrian; John M. David; Anand Giddabasappa; Amanda J. Roberts; Hernando J. Olivos; Peter J. O'Brien; Lauren Franco; Matthew W. Fields; Liliana P. Paris; Martin Friedlander; Caroline H. Johnson; Adrian A. Epstein; Howard E. Gendelman; Malcolm R. Wood; Brunhilde H. Felding; Gary J. Patti; Mary E. Spilker; Gary Siuzdak

doi:10.1038/ncomms6998

Comprehensive bioimaging with fluorinated nanoparticles using breathable liquids

Michael E. Kurczy, Zheng Jiang Zhu, Julijana Ivanisevic, Adam M. Schuyler, Kush Lalwani, Antonio F. Santidrian, John M. David, Anand Giddabasappa, Amanda J. Roberts, Hernando J. Olivos, Peter J. O'Brien, Lauren Franco, Matthew W. Fields, Liliana P. Paris, Martin Friedlander, Caroline H. Johnson, Adrian A. Epstein, Howard E. Gendelman, Malcolm R. Wood, Brunhilde H. FeldingGary J. Patti, Mary E. Spilker, Gary Siuzdak

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43 Scopus citations

Abstract

Fluorocarbons are lipophobic and non-polar molecules that exhibit remarkable biocompatibility, with applications in liquid ventilation and synthetic blood. The unique properties of these compounds have also enabled mass spectrometry imaging of tissues where the fluorocarbons act as a Teflon-like coating for nanostructured surfaces to assist in desorption/ionization. Here we report fluorinated gold nanoparticles (f-AuNPs) designed to facilitate nanostructure imaging mass spectrometry. Irradiation of f-AuNPs results in the release of the fluorocarbon ligands providing a driving force for analyte desorption. The f-AuNPs allow for the mass spectrometry analysis of both lipophilic and polar (central carbon) metabolites. An important property of AuNPs is that they also act as contrast agents for X-ray microtomography and electron microscopy, a feature we have exploited by infusing f-AuNPs into tissue via fluorocarbon liquids to facilitate multimodal (molecular and anatomical) imaging.

Original language	English (US)
Article number	5998
Journal	Nature communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms6998
State	Published - Jan 20 2015

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/ncomms6998

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Kurczy, M. E., Zhu, Z. J., Ivanisevic, J., Schuyler, A. M., Lalwani, K., Santidrian, A. F., David, J. M., Giddabasappa, A., Roberts, A. J., Olivos, H. J., O'Brien, P. J., Franco, L., Fields, M. W., Paris, L. P., Friedlander, M., Johnson, C. H., Epstein, A. A., Gendelman, H. E., Wood, M. R., ... Siuzdak, G. (2015). Comprehensive bioimaging with fluorinated nanoparticles using breathable liquids. Nature communications, 6, [5998]. https://doi.org/10.1038/ncomms6998

Kurczy, ME, Zhu, ZJ, Ivanisevic, J, Schuyler, AM, Lalwani, K, Santidrian, AF, David, JM, Giddabasappa, A, Roberts, AJ, Olivos, HJ, O'Brien, PJ, Franco, L, Fields, MW, Paris, LP, Friedlander, M, Johnson, CH, Epstein, AA, Gendelman, HE, Wood, MR, Felding, BH, Patti, GJ, Spilker, ME & Siuzdak, G 2015, 'Comprehensive bioimaging with fluorinated nanoparticles using breathable liquids', Nature communications, vol. 6, 5998. https://doi.org/10.1038/ncomms6998

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title = "Comprehensive bioimaging with fluorinated nanoparticles using breathable liquids",

abstract = "Fluorocarbons are lipophobic and non-polar molecules that exhibit remarkable biocompatibility, with applications in liquid ventilation and synthetic blood. The unique properties of these compounds have also enabled mass spectrometry imaging of tissues where the fluorocarbons act as a Teflon-like coating for nanostructured surfaces to assist in desorption/ionization. Here we report fluorinated gold nanoparticles (f-AuNPs) designed to facilitate nanostructure imaging mass spectrometry. Irradiation of f-AuNPs results in the release of the fluorocarbon ligands providing a driving force for analyte desorption. The f-AuNPs allow for the mass spectrometry analysis of both lipophilic and polar (central carbon) metabolites. An important property of AuNPs is that they also act as contrast agents for X-ray microtomography and electron microscopy, a feature we have exploited by infusing f-AuNPs into tissue via fluorocarbon liquids to facilitate multimodal (molecular and anatomical) imaging.",

author = "Kurczy, {Michael E.} and Zhu, {Zheng Jiang} and Julijana Ivanisevic and Schuyler, {Adam M.} and Kush Lalwani and Santidrian, {Antonio F.} and David, {John M.} and Anand Giddabasappa and Roberts, {Amanda J.} and Olivos, {Hernando J.} and O'Brien, {Peter J.} and Lauren Franco and Fields, {Matthew W.} and Paris, {Liliana P.} and Martin Friedlander and Johnson, {Caroline H.} and Epstein, {Adrian A.} and Gendelman, {Howard E.} and Wood, {Malcolm R.} and Felding, {Brunhilde H.} and Patti, {Gary J.} and Spilker, {Mary E.} and Gary Siuzdak",

note = "Funding Information: This work was supported by the California Institute of Regenerative Medicine grant number TR1-01219 (G.S. and M.F.), the Department of Defense (DoD) number W81XWH-13-1-0402 and ENIGMA-Ecosystems and Networks Integrated with Genes and Molecular Assemblies (http://enigma.lbl.gov), a Scientific Focus Area Program at Lawrence Berkeley National Laboratory is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Biological & Environmental Research under contract number DE-AC02-05CH11231 (G.S. and M.W.F.). It was also supported by the US National Institutes of Health numbers R01 CA170737 (B.H.F.), R24 EY017540 (G.S. and M.F.), P30 MH062261 (G.S.), RC1 HL101034 (G.S.) and P01 DA026146 (G.S.). Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited. All rights reserved.",

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doi = "10.1038/ncomms6998",

language = "English (US)",

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T1 - Comprehensive bioimaging with fluorinated nanoparticles using breathable liquids

AU - Kurczy, Michael E.

AU - Zhu, Zheng Jiang

AU - Ivanisevic, Julijana

AU - Schuyler, Adam M.

AU - Lalwani, Kush

AU - Santidrian, Antonio F.

AU - David, John M.

AU - Giddabasappa, Anand

AU - Roberts, Amanda J.

AU - Olivos, Hernando J.

AU - O'Brien, Peter J.

AU - Franco, Lauren

AU - Fields, Matthew W.

AU - Paris, Liliana P.

AU - Friedlander, Martin

AU - Johnson, Caroline H.

AU - Epstein, Adrian A.

AU - Gendelman, Howard E.

AU - Wood, Malcolm R.

AU - Felding, Brunhilde H.

AU - Patti, Gary J.

AU - Spilker, Mary E.

AU - Siuzdak, Gary

N1 - Funding Information: This work was supported by the California Institute of Regenerative Medicine grant number TR1-01219 (G.S. and M.F.), the Department of Defense (DoD) number W81XWH-13-1-0402 and ENIGMA-Ecosystems and Networks Integrated with Genes and Molecular Assemblies (http://enigma.lbl.gov), a Scientific Focus Area Program at Lawrence Berkeley National Laboratory is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Biological & Environmental Research under contract number DE-AC02-05CH11231 (G.S. and M.W.F.). It was also supported by the US National Institutes of Health numbers R01 CA170737 (B.H.F.), R24 EY017540 (G.S. and M.F.), P30 MH062261 (G.S.), RC1 HL101034 (G.S.) and P01 DA026146 (G.S.). Publisher Copyright: © 2015 Macmillan Publishers Limited. All rights reserved.

PY - 2015/1/20

Y1 - 2015/1/20

N2 - Fluorocarbons are lipophobic and non-polar molecules that exhibit remarkable biocompatibility, with applications in liquid ventilation and synthetic blood. The unique properties of these compounds have also enabled mass spectrometry imaging of tissues where the fluorocarbons act as a Teflon-like coating for nanostructured surfaces to assist in desorption/ionization. Here we report fluorinated gold nanoparticles (f-AuNPs) designed to facilitate nanostructure imaging mass spectrometry. Irradiation of f-AuNPs results in the release of the fluorocarbon ligands providing a driving force for analyte desorption. The f-AuNPs allow for the mass spectrometry analysis of both lipophilic and polar (central carbon) metabolites. An important property of AuNPs is that they also act as contrast agents for X-ray microtomography and electron microscopy, a feature we have exploited by infusing f-AuNPs into tissue via fluorocarbon liquids to facilitate multimodal (molecular and anatomical) imaging.

AB - Fluorocarbons are lipophobic and non-polar molecules that exhibit remarkable biocompatibility, with applications in liquid ventilation and synthetic blood. The unique properties of these compounds have also enabled mass spectrometry imaging of tissues where the fluorocarbons act as a Teflon-like coating for nanostructured surfaces to assist in desorption/ionization. Here we report fluorinated gold nanoparticles (f-AuNPs) designed to facilitate nanostructure imaging mass spectrometry. Irradiation of f-AuNPs results in the release of the fluorocarbon ligands providing a driving force for analyte desorption. The f-AuNPs allow for the mass spectrometry analysis of both lipophilic and polar (central carbon) metabolites. An important property of AuNPs is that they also act as contrast agents for X-ray microtomography and electron microscopy, a feature we have exploited by infusing f-AuNPs into tissue via fluorocarbon liquids to facilitate multimodal (molecular and anatomical) imaging.

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ER -

Comprehensive bioimaging with fluorinated nanoparticles using breathable liquids

Abstract

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