Computational approaches for automated classification of enzyme sequences

Akram Mohammed, Chittibabu Guda

Research output: Contribution to journalReview articlepeer-review

13 Scopus citations


Determining the functional role(s) of enzymes is very important to build the metabolic blueprint of an organism and to identify the potential roles enzymes may play in metabolic and disease pathways. With exponential growth in gene and protein sequence data, it is not feasible to experimentally characterize the function(s) of all enzymes. Alternatively, computational methods can be used to annotate the enormous amount of unannotated enzyme sequences. For function prediction and classification of enzymes, features based on amino acid composition, sequence and structural properties, domain composition and specific peptide information have been widely used by different computational approaches. Each feature space has its own merits and limitations on the overall prediction accuracy. Prediction accuracy improves when machine-learning methods are used to classify enzymes. Given the incomplete and unbalanced nature of annotations in biological databases, ensemble methods or methods that bank on a combination of orthogonal feature are more desirable for achieving higher accuracy and coverage in enzyme classification. In this review article, we systematically describe all the features and methods used thus far for enzyme class prediction. To the authors' knowledge, this review represents the most exhaustive description of methods used for computational prediction of enzyme classes.

Original languageEnglish (US)
Pages (from-to)147-152
Number of pages6
JournalJournal of Proteomics and Bioinformatics
Issue number8
StatePublished - 2011


  • Amino acid composition
  • Domain composition
  • Ensemble method
  • Enzyme classification
  • Machine learning
  • Nearest neighbor predictor
  • Sequence similarity
  • Structural information
  • Support vector machine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Cell Biology


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