Concise review: Non-cell autonomous reprogramming: A nucleic acid-free approach to induction of pluripotency

Sowmya Parameswaran, Sudha Balasubramanian, Mahendra S. Rao, Iqbal Ahmad

Research output: Contribution to journalReview articlepeer-review

8 Scopus citations

Abstract

The reprogramming of somatic cells to a pluripotent state by the expression of a defined set of exogenous transcription factors represents a significant breakthrough for the use of stem cells in regenerative medicine. It has the potential to make autologous stem cell therapy practical and promote better understanding of the disease processes by generating patient specific stem cells. Several strategies have been used to generate induced pluripotent stem cells (iPSCs) that include nucleic acid and non-nucleic acidbased approaches, with and without epigenetic modifications. The purpose of these different approaches for generating iPSCs, besides understanding the underlying mechanism, is to develop a facile method for reprogramming without genetic alteration, suitable for clinical use. Here, we discuss different strategies for generating iPSCs, with an emphasis on a recent non-cell autonomous approach to reprogram somatic progenitors that regenerate cornea to a pluripotent state through the recruitment of endogenous transcription factors.

Original languageEnglish (US)
Pages (from-to)1013-1020
Number of pages8
JournalSTEM CELLS
Volume29
Issue number7
DOIs
StatePublished - Jul 2011

Keywords

  • Cornea
  • Induced pluripotency
  • Induced pluripotent stem cells
  • Limbal stem cells
  • Niche
  • Non-cell autonomous
  • Reprogramming

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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