Confinement-induced quorum sensing of individual Staphylococcus aureus bacteria

Eric C. Carnes, Deanna M. Lopez, Niles P. Donegan, Ambrose Cheung, Hattie Gresham, Graham S. Timmins, C. Jeffrey Brinker

Research output: Contribution to journalArticlepeer-review

150 Scopus citations

Abstract

It is postulated that in addition to cell density, other factors such as the dimensions and diffusional characteristics of the environment could influence quorum sensing (QS) and induction of genetic reprogramming. Modeling studies predict that QS may operate at the level of a single cell, but, owing to experimental challenges, the potential benefits of QS by individual cells remain virtually unexplored. Here we report a physical system that mimics isolation of a bacterium, such as within an endosome or phagosome during infection, and maintains cell viability under conditions of complete chemical and physical isolation. For Staphylococcus aureus, we show that quorum sensing and genetic reprogramming can occur in a single isolated organism. Quorum sensing allows S. aureus to sense confinement and to activate virulence and metabolic pathways needed for survival. To demonstrate the benefit of confinement-induced quorum sensing to individuals, we showed that quorum-sensing bacteria have significantly greater viability over non-QS bacteria.

Original languageEnglish (US)
Pages (from-to)41-45
Number of pages5
JournalNature Chemical Biology
Volume6
Issue number1
DOIs
StatePublished - Jan 2010
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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