Conjugative transfer in staphylococcus aureus

Cortney R. Halsey, Paul D Fey

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The acquisition of plasmids has led to a significant increase in antimicrobial resistance within the staphylococci. In order to study these plasmids effectively, one must be able move the plasmid DNA into genetically clean backgrounds. While the smaller staphylococcal class I (1-5 kb) and class II (10-30 kb) plasmids are readily transferred using bacteriophage transduction or electroporation, these methods are inefficient at moving the larger class III (30-60 kb) plasmids. This review describes methods to transfer class III plasmids via conjugative mobilization.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages83-87
Number of pages5
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1373
ISSN (Print)1064-3745

Keywords

  • Conjugation
  • Plasmid
  • Staphylococci

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Halsey, C. R., & Fey, P. D. (2016). Conjugative transfer in staphylococcus aureus. In Methods in Molecular Biology (pp. 83-87). (Methods in Molecular Biology; Vol. 1373). Humana Press Inc.. https://doi.org/10.1007/7651_2015_276