Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene

Goumin Chen, Shuhui Wang, Kun Xiong, Jinzhong Wang, Tao Ye, Wenping Dong, Qi Wang, Qimin Chen, Yunqi Geng, Charles Wood, Yi Zeng

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

HIV-1HXB2 5'LTR region, most of BIVR29 gag-pol segment and HIV-1HXB2 pol IN - 3'LTR region were respectively amplified. A chimeric clone, designated as pHBIV3753, was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene.

Original languageEnglish (US)
Pages (from-to)123-125
Number of pages3
JournalAIDS
Volume16
Issue number1
DOIs
StatePublished - Jan 4 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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    Chen, G., Wang, S., Xiong, K., Wang, J., Ye, T., Dong, W., Wang, Q., Chen, Q., Geng, Y., Wood, C., & Zeng, Y. (2002). Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene. AIDS, 16(1), 123-125. https://doi.org/10.1097/00002030-200201040-00016