Construction and use of a template block for radial immunodiffusion

Thomas L. McDonald; Annika Weber; Geoffrey Thiele

doi:10.1016/0003-2697(90)90591-V

Construction and use of a template block for radial immunodiffusion

Thomas L. McDonald, Annika Weber, Geoffrey Thiele

Research output: Contribution to journal › Article

1 Scopus citations

Abstract

Details for design and construction of template blocks for the increased sensitivity of radial immunodiffusion (RID) assays are described. The sensitivity of the template RID was 10-fold higher for quantifying albumin and 5-fold higher for IgG than the conventional RID with wells cut into the agarose. The system described in this communication resulted from the unit which provided the most consistent results from a variety of trials with different block thicknesses, chamber sizes and depths, and chamber arrangements.

Original language	English (US)
Pages (from-to)	165-168
Number of pages	4
Journal	Analytical Biochemistry
Volume	186
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(90)90591-V
State	Published - Apr 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Details for design and construction of template blocks for the increased sensitivity of radial immunodiffusion (RID) assays are described. The sensitivity of the template RID was 10-fold higher for quantifying albumin and 5-fold higher for IgG than the conventional RID with wells cut into the agarose. The system described in this communication resulted from the unit which provided the most consistent results from a variety of trials with different block thicknesses, chamber sizes and depths, and chamber arrangements.",

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