Construction of a live-attenuated HIV-1 vaccine through genetic code expansion

Nanxi Wang, Yue Li, Wei Niu, Ming Sun, Ronald Cerny, Qingsheng Li, Jiantao Guo

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

A safe and effective vaccine against human immunodeficiency virus type 1 (HIV-1) is urgently needed to combat the worldwide AIDS pandemic, but still remains elusive. The fact that uncontrolled replication of an attenuated vaccine can lead to regaining of its virulence creates safety concerns precluding many vaccines from clinical application. We introduce a novel approach to control HIV-1 replication, which entails the manipulation of essential HIV-1 protein biosynthesis through unnatural amino acid (UAA*)-mediated suppression of genome-encoded blank codon. We successfully demonstrate that HIV-1 replication can be precisely turned on and off in vitro.

Original languageEnglish (US)
Pages (from-to)4867-4871
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number19
DOIs
StatePublished - May 5 2014

Keywords

  • HIV-1 vaccine
  • attenuated vaccine
  • genetic code expansion
  • unnatural amino acid
  • virus engineering

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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