Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus

Jennifer L. Endres, Vijaya Kumar Yajjala, Paul D. Fey, Kenneth W. Bayles

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Transposon mutagenesis is one of the most widely used strategies to generate a large number of random mutations within a bacterial genome and then to precisely identify the mutated sites. The generation of sequence-defined transposon mutant libraries that are composed of a collection of different mutants, each containing a single transposon insertion mutation within nearly all of the nonessential genes within the genome, is a rapid and reliable way to enhance the study of gene function. In this chapter, we describe the process to generate a sequence-defined transposon mutant library in Staphylococcus aureus utilizing the mariner-based bursa aurealis transposon.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Random mutagenesis
  • Staphylococcus aureus
  • Transposon
  • bursa aurealis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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