@inbook{1df60fd149ba4ab28c64204bebcd6a47,
title = "Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus",
abstract = "Transposon mutagenesis is one of the most widely used strategies to generate a large number of random mutations within a bacterial genome and then to precisely identify the mutated sites. The generation of sequence-defined transposon mutant libraries that are composed of a collection of different mutants, each containing a single transposon insertion mutation within nearly all of the nonessential genes within the genome, is a rapid and reliable way to enhance the study of gene function. In this chapter, we describe the process to generate a sequence-defined transposon mutant library in Staphylococcus aureus utilizing the mariner-based bursa aurealis transposon.",
keywords = "Random mutagenesis, Staphylococcus aureus, Transposon, bursa aurealis",
author = "Endres, {Jennifer L.} and Yajjala, {Vijaya Kumar} and Fey, {Paul D.} and Bayles, {Kenneth W.}",
year = "2019",
doi = "10.1007/978-1-4939-9570-7_3",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "29--37",
booktitle = "Methods in Molecular Biology",
}