Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus

Jennifer L. Endres, Vijaya Kumar Yajjala, Paul D. Fey, Kenneth W. Bayles

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Transposon mutagenesis is one of the most widely used strategies to generate a large number of random mutations within a bacterial genome and then to precisely identify the mutated sites. The generation of sequence-defined transposon mutant libraries that are composed of a collection of different mutants, each containing a single transposon insertion mutation within nearly all of the nonessential genes within the genome, is a rapid and reliable way to enhance the study of gene function. In this chapter, we describe the process to generate a sequence-defined transposon mutant library in Staphylococcus aureus utilizing the mariner-based bursa aurealis transposon.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages29-37
Number of pages9
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume2016
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Random mutagenesis
  • Staphylococcus aureus
  • Transposon
  • bursa aurealis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Endres, J. L., Yajjala, V. K., Fey, P. D., & Bayles, K. W. (2019). Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus. In Methods in Molecular Biology (pp. 29-37). (Methods in Molecular Biology; Vol. 2016). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9570-7_3