Continuous chromogenic tissue factor assay: Comparison to clot-based assays and sensitivity established using pure tissue factor

Steven D. Carson

doi:10.1016/0049-3848(87)90453-1

Continuous chromogenic tissue factor assay: Comparison to clot-based assays and sensitivity established using pure tissue factor

Steven D. Carson

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

The continuous chromogenic tissue factor assay has been compared to one-stage and two-stage coagulation assays, and its sensitivity has been established using purified human placental tissue factor. Loglog plots of the clot-times versus A405/min2 are linear over the mutually useful ranges of tissue factor concentration. Using human placental tissue factor reconstituted into phosphatidylserine-phosphatidylcholine (30:70) vesicles, the chromogenic assay quantitatively detected tissue factor in dilutions containing from 3 femtomoles to less than 10 attomoles of protein.

Original language	English (US)
Pages (from-to)	379-387
Number of pages	9
Journal	Thrombosis Research
Volume	47
Issue number	4
DOIs	https://doi.org/10.1016/0049-3848(87)90453-1
State	Published - Aug 15 1987
Externally published	Yes

Keywords

chromogenic assay
coagulation assays
tissue factor

ASJC Scopus subject areas

Hematology

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10.1016/0049-3848(87)90453-1

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title = "Continuous chromogenic tissue factor assay: Comparison to clot-based assays and sensitivity established using pure tissue factor",

abstract = "The continuous chromogenic tissue factor assay has been compared to one-stage and two-stage coagulation assays, and its sensitivity has been established using purified human placental tissue factor. Loglog plots of the clot-times versus A405/min2 are linear over the mutually useful ranges of tissue factor concentration. Using human placental tissue factor reconstituted into phosphatidylserine-phosphatidylcholine (30:70) vesicles, the chromogenic assay quantitatively detected tissue factor in dilutions containing from 3 femtomoles to less than 10 attomoles of protein.",

keywords = "chromogenic assay, coagulation assays, tissue factor",

author = "Carson, {Steven D.}",

note = "Funding Information: This research was supported by grant HL-31408 from NIH, a grant-in-aid from the Colorado Heart Association, and by R .J . Reynolds-Nabisco .",

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doi = "10.1016/0049-3848(87)90453-1",

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T1 - Continuous chromogenic tissue factor assay

T2 - Comparison to clot-based assays and sensitivity established using pure tissue factor

AU - Carson, Steven D.

N1 - Funding Information: This research was supported by grant HL-31408 from NIH, a grant-in-aid from the Colorado Heart Association, and by R .J . Reynolds-Nabisco .

PY - 1987/8/15

Y1 - 1987/8/15

N2 - The continuous chromogenic tissue factor assay has been compared to one-stage and two-stage coagulation assays, and its sensitivity has been established using purified human placental tissue factor. Loglog plots of the clot-times versus A405/min2 are linear over the mutually useful ranges of tissue factor concentration. Using human placental tissue factor reconstituted into phosphatidylserine-phosphatidylcholine (30:70) vesicles, the chromogenic assay quantitatively detected tissue factor in dilutions containing from 3 femtomoles to less than 10 attomoles of protein.

AB - The continuous chromogenic tissue factor assay has been compared to one-stage and two-stage coagulation assays, and its sensitivity has been established using purified human placental tissue factor. Loglog plots of the clot-times versus A405/min2 are linear over the mutually useful ranges of tissue factor concentration. Using human placental tissue factor reconstituted into phosphatidylserine-phosphatidylcholine (30:70) vesicles, the chromogenic assay quantitatively detected tissue factor in dilutions containing from 3 femtomoles to less than 10 attomoles of protein.

KW - chromogenic assay

KW - coagulation assays

KW - tissue factor

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JF - Thrombosis Research

IS - 4

ER -

Continuous chromogenic tissue factor assay: Comparison to clot-based assays and sensitivity established using pure tissue factor

Abstract

Keywords

ASJC Scopus subject areas

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