Continuous treatment with cisplatin induces the oocyte death of primordial follicles without activation

Maya Eldani, Yi Luan, Pauline C. Xu, Tom Bargar, So Youn Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Chemotherapy directly or indirectly affects organs in a short-term or continuous manner. Endocrine organs are especially sensitive to cancer treatment, leading to concerns among patients regarding their quality of life afterward. Side effects to the ovary include damage to the ovarian reserve, resulting in follicle loss, endocrine hormone deficiency, and infertility. It has been previously demonstrated that continuous treatment with 2 mg/kg cisplatin for 15 days can activate primordial follicles, suggesting that the response in the oocytes of primordial follicles was dependent on cisplatin concentration and administration frequency. However, our results demonstrate that continuous treatment with 2 mg/kg cisplatin for 15 days leads to the same consequence as with the continuous treatment of 5 mg/kg cisplatin: the death of oocytes in primordial follicles without indication of activation. Moreover, animals co-injected with melatonin and cisplatin did not display any significant differences from those treated with cisplatin only contrary to the known results. 6-hydroxymelatonin, a metabolite of melatonin, could not prevent follicle destruction, implying that melatonin does not confer the protection of ovarian follicles, either directly or indirectly. Altogether, our data support that fertoprotectants against cisplatin must target molecules that control cell death pathways in the oocytes of primordial follicles.

Original languageEnglish (US)
Pages (from-to)13885-13899
Number of pages15
JournalFASEB Journal
Volume34
Issue number10
DOIs
StatePublished - Oct 1 2020

Keywords

  • apoptosis
  • cisplatin
  • dose
  • melatonin
  • ovary

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Continuous treatment with cisplatin induces the oocyte death of primordial follicles without activation'. Together they form a unique fingerprint.

Cite this