@article{c8e836478917495880bb8acd3783d6ef,
title = "Converting 2D Nanofiber Membranes to 3D Hierarchical Assemblies with Structural and Compositional Gradients Regulates Cell Behavior",
abstract = "New methods are described for converting 2D electrospun nanofiber membranes to 3D hierarchical assemblies with structural and compositional gradients. Pore-size gradients are generated by tuning the expansion of 2D membranes in different layers with incorporation of various amounts of a surfactant during the gas-foaming process. The gradient in fiber organizations is formed by expanding 2D nanofiber membranes composed of multiple regions collected by varying rotating speeds of mandrel. A compositional gradient on 3D assemblies consisting of radially aligned nanofibers is prepared by dripping, diffusion, and crosslinking. Bone mesenchymal stem cells (BMSCs) on the 3D nanofiber assemblies with smaller pore size show significantly higher expression of hypoxia-related markers and enhanced chondrogenic differentiation compared to BMSCs cultured on the assemblies with larger pore size. The basic fibroblast growth factor gradient can accelerate fibroblast migration from the surrounding area to the center in an in vitro wound healing model. Taken together, 3D nanofiber assemblies with gradients in pore sizes, fiber organizations, and contents of signaling molecules can be used to engineer tissue constructs for tissue repair and build biomimetic disease models for studying disease biology and screening drugs, in particular, for interface tissue engineering and modeling.",
keywords = "3D nanofiber assemblies, composition, fiber organization, gradients, pore size",
author = "Shixuan Chen and Alec McCarthy and John, {Johnson V.} and Yajuan Su and Jingwei Xie",
note = "Funding Information: This work was partially supported by startup funds from the University of Nebraska Medical Center (UNMC), National Institute of General Medical Science (NIGMS) and National Institute of Dental and Craniofacial Research (NIDCR) of the National Institutes of Health under Award Numbers R01GM123081 and 1R21DE027516, Congressionally Directed Medical Research Program (CDMRP)/Peer Reviewed Medical Research Program (PRMRP) under FY19 W81XWH2010207, UNMC Regenerative Medicine Program pilot grant, Nebraska Research Initiative grant, and NE LB606. BMSCs were isolated from a 6-week-old male Sprague–Dawley rat using a standard protocol. Cells were used for in vitro studies before passage 4. The isolation of BMSCs from rat was approved by IACUC at the University of Nebraska Medical Center (protocol ID: 15-067-09-FC). The GFP-labeled human dermal fibroblasts used in this work were a gift from Dr. Mark A. Carlson's laboratory at the University of Nebraska Medical Center. Funding Information: This work was partially supported by startup funds from the University of Nebraska Medical Center (UNMC), National Institute of General Medical Science (NIGMS) and National Institute of Dental and Craniofacial Research (NIDCR) of the National Institutes of Health under Award Numbers R01GM123081 and 1R21DE027516, Congressionally Directed Medical Research Program (CDMRP)/Peer Reviewed Medical Research Program (PRMRP) under FY19 W81XWH2010207, UNMC Regenerative Medicine Program pilot grant, Nebraska Research Initiative grant, and NE LB606. BMSCs were isolated from a 6‐week‐old male Sprague–Dawley rat using a standard protocol. Cells were used for in vitro studies before passage 4. The isolation of BMSCs from rat was approved by IACUC at the University of Nebraska Medical Center (protocol ID: 15‐067‐09‐FC). The GFP‐labeled human dermal fibroblasts used in this work were a gift from Dr. Mark A. Carlson's laboratory at the University of Nebraska Medical Center. Publisher Copyright: {\textcopyright} 2020 Wiley-VCH GmbH",
year = "2020",
month = oct,
day = "1",
doi = "10.1002/adma.202003754",
language = "English (US)",
volume = "32",
journal = "Advanced Materials",
issn = "0935-9648",
publisher = "Wiley-Blackwell",
number = "43",
}