Copy number of the 16S rRNA gene in Rickettsia prowazekii

H. Pang, H. H. Winkler

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The obligate intracellular parasite, Rickettsia prowazekii, is a slowly growing bacterium with a doubling time of 8 to 12 h. The copy number of the 16S rRNA gene in the rickettsial chromosome was determined to be one. Genomic DNA from R. prowazekii was digested either by a variety of restriction enzymes known not to cut at any site in the rickettsial 16S rRNA gene or by a combination of these noncutting enzymes and SmaI, which cuts the gene only once. Only one DNA fragment in these digests hybridized to a biotinylated probe containing a portion of the rickettsial 16S rRNA gene. Moreover, the density of the rickettsial 16S rRNA gene fragment after hybridization was equal to the density of each of the seven 16S rRNA gene fragments in Escherichia coli.

Original languageEnglish (US)
Pages (from-to)3893-3896
Number of pages4
JournalJournal of bacteriology
Volume175
Issue number12
DOIs
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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