Abstract
CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail or earpiece DNA, are unavailable. We develop CRISPR-KRISPR (CRISPR-Knock-ins and Random Inserts Searching PRotocol), a method to identify both off-target lesions and random insertions. CRISPR-KRISPR uses as little as 3.4 μg of genomic DNA; thus, it can be easily incorporated as an additional step to genotype founder animals for further breeding.
Original language | English (US) |
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Article number | 228 |
Journal | Genome biology |
Volume | 23 |
Issue number | 1 |
DOIs | |
State | Published - Dec 2022 |
Keywords
- CIRCLE-seq
- CRISPR
- CRISPR-KRISPR
- Donor DNA
- Intronic region
- Knock-in
- Off target
- Random insertion
- Repeat element
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Cell Biology