@article{17a3470026f74accb504e650e560aa56,
title = "CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice",
abstract = "CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail or earpiece DNA, are unavailable. We develop CRISPR-KRISPR (CRISPR-Knock-ins and Random Inserts Searching PRotocol), a method to identify both off-target lesions and random insertions. CRISPR-KRISPR uses as little as 3.4 μg of genomic DNA; thus, it can be easily incorporated as an additional step to genotype founder animals for further breeding.",
keywords = "CIRCLE-seq, CRISPR, CRISPR-KRISPR, Donor DNA, Intronic region, Knock-in, Off target, Random insertion, Repeat element",
author = "Masayuki Tanaka and Keiko Yokoyama and Hideki Hayashi and Sanae Isaki and Kanae Kitatani and Ting Wang and Hisako Kawata and Hideyuki Matsuzawa and Gurumurthy, {Channabasavaiah B.} and Hiromi Miura and Masato Ohtsuka",
note = "Funding Information: We thank Nick May, TypeRight for copy-editing of the manuscript and Joe Miano and Bart Williams, Augusta University, for feedback on the manuscript. Kevin Pang was the primary editor of this article and managed its editorial process and peer review in collaboration with the rest of the editorial team. The review history is available as Additional file 2. Funding Information: This work was supported in part by the Research and Study Project of Tokai University General Research Organization (2016-2018), 2016-2017 Tokai University School of Medicine Project Research to M.O. C.B.G acknowledges the funding support from the NIH (awards R35HG010719 and R21GM129559). Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1186/s13059-022-02779-8",
language = "English (US)",
volume = "23",
journal = "Genome biology",
issn = "1474-7596",
publisher = "BioMed Central",
number = "1",
}