CRISPR/Cas9 as a Mutagenic Factor

Andrey R. Shumega, Youri I. Pavlov, Angelina V. Chirinskaite, Aleksandr A. Rubel, Sergey G. Inge-Vechtomov, Elena I. Stepchenkova

Research output: Contribution to journalReview articlepeer-review


The discovery of the CRISPR/Cas9 microbial adaptive immune system has revolutionized the field of genetics, by greatly enhancing the capacity for genome editing. CRISPR/Cas9-based editing starts with DNA breaks (or other lesions) predominantly at target sites and, unfortunately, at off-target genome sites. DNA repair systems differing in accuracy participate in establishing desired genetic changes but also introduce unwanted mutations, that may lead to hereditary, oncological, and other diseases. New approaches to alleviate the risks associated with genome editing include attenuating the off-target activity of editing complex through the use of modified forms of Cas9 nuclease and single guide RNA (sgRNA), improving delivery methods for sgRNA/Cas9 complex, and directing DNA lesions caused by the sgRNA/Cas9 to non-mutagenic repair pathways. Here, we have described CRISPR/Cas9 as a new powerful mutagenic factor, discussed its mutagenic properties, and reviewed factors influencing the mutagenic activity of CRISPR/Cas9.

Original languageEnglish (US)
Article number823
JournalInternational journal of molecular sciences
Issue number2
StatePublished - Jan 2024


  • CRISPR/Cas9
  • genome editing
  • mutations
  • off-target activity

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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